Effects of glucose variability on hematopoietic stem/progenitor cells in patients with type 1 diabetes
| Autor: | Angelo Avogaro, Benedetta Maria Bonora, Marianna D’Anna, Silvia Tresso, Roberta Cappellari, G. P. Fadini, Daniela Bruttomesso, Federico Boscari |
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| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Continuous glucose monitoring Endothelial progenitor cells Flash glucose monitoring system Glucose variability Hematopoietic stem/progenitor cells Type 1 diabetes endocrine system diseases Endocrinology Diabetes and Metabolism CD34 Adrenergic 030209 endocrinology & metabolism Bone Marrow Cells Flow cytometry 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine Diabetes mellitus medicine Humans Progenitor cell Tissue homeostasis Cells Cultured medicine.diagnostic_test business.industry nutritional and metabolic diseases Middle Aged medicine.disease Hematopoietic Stem Cells Prognosis Haematopoiesis Diabetes Mellitus Type 1 Glucose 030220 oncology & carcinogenesis Case-Control Studies Female business Follow-Up Studies |
| Zdroj: | Journal of endocrinological investigation. 44(1) |
| ISSN: | 1720-8386 |
| Popis: | Diabetes reduces the levels of hematopoietic stem/progenitor cells (HSPCs), which can contribute to organ and tissue homeostasis. Among patients with diabetes, lower HSPC levels predict the development or worsening of micro- and macro-angiopathy. High glucose variability is also associated with diabetic complications and we have previously shown that acute hypoglycaemia can stimulate stem/progenitor cells. Thus, we evaluated the relationship between glucose variability or time in hypoglycaemia and HSPCs in patients with type 1 diabetes (T1D). Patients with T1D were compared to healthy subjects. HSPCs (CD34+, CD133+, CD34+CD133+, CD34 + CD45dim) were quantified by flow cytometry. Using flash glucose monitoring system for 90 days, we calculated several measures of glucose variability and time in hypoglycaemia. Forty-four patients with T1D and 44 healthy subjects were enrolled. Compared to healthy controls, T1D patients had significantly lower levels of HSPCs and duration of diabetes was inversely correlated with HSPC levels. Significant direct correlations were found between HSPC levels and the coefficient of variation of glucose levels or time in hypoglycaemia, which were stronger in patients with short-term than in those with long-standing diabetes. This study confirms the pauperization of HSPCs in T1D patients and demonstrates a potential HSPC-stimulatory effect of hypoglycaemia, which mitigates with long-lasting diabetes. These data are consistent with a model whereby disease chronicity progressively blunts the release of HSPCs in response to adrenergic triggers, like hypoglycaemic events. |
| Databáze: | OpenAIRE |
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