Phytochemical Composition, Antioxidant, and Antimicrobial Attributes of Different Solvent Extracts from Myrica esculenta Buch.-Ham. ex. D. Don Leaves
| Autor: | Atul Kabra, Rohit Sharma, Christophe Hano, Ruchika Kabra, Uttam Singh Baghel, Natália Martins |
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| Přispěvatelé: | Inder Kumar Gujral Punjab Technical University, Partenaires INRAE, Kota College of Pharmacy, The Central Council for Research in Ayurvedic Sciences, Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Institut National de la Recherche Agronomique (INRA)-Université d'Orléans (UO), Universidade do Porto, University of Kota, Instituto de Investigação e Inovação em Saúde |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Myrica esculenta Antifungal Agents antioxidant activité anti-microbienne 030309 nutrition & dietetics DPPH Phytochemicals Ethyl acetate lcsh:QR1-502 01 natural sciences Biochemistry Antioxidants lcsh:Microbiology Gram-Negative Bacteria / drug effects Biphenyl Compounds / antagonists & inhibitors chemistry.chemical_compound Phenolic composition Candida albicans Anti-Bacterial Agents / chemistry Antifungal Agents / isolation & purification Gallic acid Antioxidants / chemistry Myrica / chemistry Plant Extracts / isolation & purification 0303 health sciences ABTS Phytochemicals / chemistry biology activité antioxydante Solvents / chemistry Anti-Bacterial Agents / pharmacology Benzothiazoles / antagonists & inhibitors Anti-Bacterial Agents Antifungal Agents / pharmacology Sulfonic Acids / antagonists & inhibitors Picrates / antagonists & inhibitors Aspergillus niger Aspergillus niger / drug effects Antioxidant Phytochemicals / isolation & purification Quercetin composé phytochimique Autre (Sciences du Vivant) Antioxidants / isolation & purification [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] Plant Extracts / chemistry composé phénolique Food Contamination Anti-Bacterial Agents / isolation & purification Candida albicans / drug effects Gram-Positive Bacteria activité anti-oxydante Article Antifungal Agents / chemistry antimicrobial phenolic composition 03 medical and health sciences Picrates Gram-Negative Bacteria Benzothiazoles Phenols Gram-Positive Bacteria / drug effects Molecular Biology Chromatography Plant Extracts Antioxidants / pharmacology Food Contamination / analysis Biphenyl Compounds biology.organism_classification Myrica Phytochemicals / pharmacology Plant Extracts / pharmacology chemistry Polyphenol Solvents flavonoïde Antimicrobial Sulfonic Acids human activities 010606 plant biology & botany |
| Zdroj: | Biomolecules 8 (9), 357. (2019) Biomolecules, Vol 9, Iss 8, p 357 (2019) Biomolecules Biomolecules, MDPI, 2019, 9 (8), pp.357. ⟨10.3390/biom9080357⟩ Volume 9 Issue 8 |
| ISSN: | 2218-273X |
| DOI: | 10.3390/biom9080357⟩ |
| Popis: | Background: Plant diversity is a basic source of food and medicine for local Himalayan communities. The current study was designed to assess the effect of different solvents (methanol, ethyl acetate, and water) on the phenolic profile, and the corresponding biological activity was studied. Methods: Antioxidant activity was investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&Prime azino-bis(3-ethylbenzothiazoline-6-sulphonic) acid (ABTS) assay, while the antimicrobial activity was evaluated by disk diffusion method using various bacterial and fungal strains. Results: The outcomes demonstrated that methanol acted as the most effective solvent for polyphenols extraction, as strengthened by the liquid chromatography and mass spectroscopy (LC-MS) and fourier transform infrared spectroscopy (FTIR) analysis. M. esculenta methanol extract showed the highest DPPH and ABTS radical scavenger antioxidant activity with IC50 values of 39.29 &mu g/mL and 52.83 &mu g/mL, respectively, while the ethyl acetate and aqueous extracts revealed minimum antioxidant potential. Methanol extract also revealed higher phenolic content, 88.94± 0.24 mg of equivalent gallic acid (GAE)/g), measured by the Folin&ndash Ciocalteu method, while the minimum content was recorded for aqueous extract (62.38± 0.14 GAE/g). The highest flavonoid content was observed for methanol extract, 67.44± 0.14 mg quercetin equivalent (QE)/g) measured by an aluminum chloride colorimetric method, while the lowest content was recorded for aqueous extract (35.77± 0.14 QE/g). Antimicrobial activity findings also reveal that the methanol extract led to a higher inhibition zone against bacterial and fungal strains. FTIR analysis reveals the presence of various functional groups, viz. alkenes, amines, carboxylic acids, amides, esters, alcohols, phenols, ketones, carboxylic acids, and aromatic compounds. This FTIR analysis could serve as a basis for the authentication of M. esculenta extracts for future industrial applications. Compounds identified by LC-MS analysis were gallic acid, myricanol, myricanone, epigallocatechin 3-O-gallate, &beta sitosterol, quercetin, p-coumaric acid, palmitic acid, n-pentadecanol, n-octadecanol, stigmasterol, oleanolic acid, n-hexadecanol, cis-&beta caryophyllene, lupeol, and myresculoside. Conclusion: This study suggests that the methanolic extract from M. esculenta leaves has strong antioxidant potential and could be a significant source of natural antioxidants and antimicrobials for functional foods formulation. |
| Databáze: | OpenAIRE |
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