The Horse Chestnut Lines Harboring the rol Genes

Autor: P.H. Druart, Lj. Radojević, Snežana Zdravković-Korać, Dušica Ćalić
Rok vydání: 2003
Předmět:
Zdroj: Biologia Plantarum
ISSN: 1573-8264
0006-3134
DOI: 10.1023/b:biop.0000041051.81210.9b
Popis: An Agrobacterium rhizogenes-mediated transformation system for Aesculus hippocastanum L. has been developed. Wounded androgenic embryos of A. hippocastanum were inoculated with bacteria containing the pRiA4 plasmid, with the uid A sequence as a reporter gene. The hairy roots emerging from the wounded sites of androgenic embryos were isolated and maintained in Murashige and Skoog's (MS) liquid hormone-free medium. Five hairy root lines have been maintained in vitro for 4 years with unchanged growth rate and might be a suitable source for secondary metabolite production. The transformation events have been confirmed by a polymerase chain reaction specific to the rol A, B, C and D genes. The absence of residual contaminating bacteria has been shown by a polymerase chain reaction specific to the vir D1 sequence. null
Databáze: OpenAIRE
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