Citrulline enhances myofibrillar constituents expression of skeletal muscle and induces a switch in muscle energy metabolism in malnourished aged rats

Autor: Béatrice Morio, Philippe Chafey, Servane Le Plénier, Christophe Moinard, Voahangy Randrianarison-Huetz, C. Faure, Christian Aussel, Philippe Noirez, Luc Cynober
Přispěvatelé: Prévention et traitement de la perte protéique musculaire en situation de résistance à l'anabolisme (PRETRRAM - EA 4466), Université Paris Descartes - Paris 5 (UPD5), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Plateforme protéomique 3P5 [Institut Cochin] (3P5), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), UFR STAPS, service interhospitalier de Biochimie, Hôpital de l'Hotel Dieu, A- P H-P, service biologie, Centre Hospitalier Emile Roux [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Stress Cellulaire : Physiopathologie, Stratégies Nutritionnelles et Thérapeutiques Innovantes (EA 4466), Université Paris Descartes - Paris 5 (UPD5)-Faculté des Sciences Pharmaceutiques et Biologiques, APPERT Institute, Fondation Le Lous, Clermont Université-Université d'Auvergne - Clermont-Ferrand I (UdA)-Institut National de la Recherche Agronomique (INRA), Centre Hospitalier Emile Roux, Prévention et traitement de la perte protéique musculaire en situation de résistance à l'anabolisme ( PRETRRAM - EA 4466 ), Université Paris Descartes - Paris 5 ( UPD5 ), Unité de Nutrition Humaine ( UNH ), Institut National de la Recherche Agronomique ( INRA ) -Université d'Auvergne - Clermont-Ferrand I ( UdA ) -Clermont Université, Plateforme protéomique 3P5 [Institut Cochin] ( 3P5 ), Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Stress Cellulaire : Physiopathologie, Stratégies Nutritionnelles et Thérapeutiques Innovantes ( EA 4466 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Faculté des Sciences Pharmaceutiques et Biologiques, Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Male
Proteomics
Glycogenolysis
Arginine
Proteome
030309 nutrition & dietetics
[ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition
Muscle Proteins
arginine
Biochemistry
Rats
Sprague-Dawley
Random Allocation
chemistry.chemical_compound
Citrulline
Glycolysis
Myofibrillar proteins
myosin heavy-chain
2. Zero hunger
0303 health sciences
dysfunction
Energetic metabolism
protein-synthesis
Animal proteomics
Mitochondrial respiratory chain
medicine.anatomical_structure
Muscle
leucine
medicine.medical_specialty
proteolysis
Biology
03 medical and health sciences
atrophy
Internal medicine
medicine
Animals
Muscle
Skeletal
Molecular Biology
030304 developmental biology
Skeletal muscle
Metabolism
gel-electrophoresis
Diet
Rats
Citric acid cycle
Endocrinology
chemistry
identification
Energy Metabolism
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
massive intestinal resection
Zdroj: Proteomics
Proteomics, 2013, 13 (14), pp.2191-2201. ⟨10.1002/pmic.201200262⟩
Proteomics, Wiley-VCH Verlag, 2013, 13 (14), pp.2191-2201. ⟨10.1002/pmic.201200262⟩
Proteomics, Wiley-VCH Verlag, 2013, 13 (14), pp.2191-2201. 〈10.1002/pmic.201200262〉
ISSN: 1615-9853
1615-9861
DOI: 10.1002/pmic.201200262⟩
Popis: We are grateful to Florence Blandel for carbonylated protein analysis. We would like to thank Morgane Le-Gall, Guilhem Clary, and Cedric Broussard for their help in the DIGE experiments and MS analysis. We also thank Professor E. Fontaine for helpful discussion.; Citrulline (Cit) actions on muscle metabolism remain unclear. Those latter were investigated using a proteomic approach on Tibialis muscles from male Sprague-Dawley rats. At 23 months of age, rats were either fed ad libitum (AL group) or subjected to dietary restriction for 12 weeks. At the end of the restriction period, one group of rats was euthanized (R group) and two groups were refed for one week with a standard diet supplemented with nonessential amino acids group or Cit (CIT group). Results of the proteomic approach were validated using targeted Western blot analysis and assessment of gene expression of the related genes. Maximal activities of the key enzymes involved in mitochondrial functioning were also determined. Cit supplementation results in a significant increase in the protein expression of the main myofibrillar constituents and of a few enzymes involved in glycogenolysis and glycolysis (CIT vs. AL and R, p < 0.05). Conversely, the expression of oxidative enzymes from Krebs cycle and mitochondrial respiratory chain was significantly decreased (CIT vs. AL, p < 0.05). However, maximal activities of key enzymes of mitochondrial metabolism were not significantly affected, except for complex 1 which presented an increased activity (CIT vs. AL and R, p < 0.05). In conclusion, Cit supplementation increases expression of the main myofibrillar proteins and seems to induce a switch in muscle energy metabolism, from aerobia toward anaerobia.
Databáze: OpenAIRE
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