Kallikrein-related peptidase 7 is a potential target for the treatment of pancreatic cancer
| Autor: | Ding Zhang, Xiao Tan, Wei Hong Zheng, Wei Liu, Sen Liu, Ru Cheng Yao, Jun Zheng, Xiao-Song Li, Lin Li, Jian Ping Du, Fangyu Wang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment pancreatic ductal adenocarcinoma Disease Small hairpin RNA 03 medical and health sciences 0302 clinical medicine kallikrein-related peptidase 7 Pancreatic cancer KLK7 medicine short hairpin RNA small organic inhibitor Survival rate Chemotherapy Cell growth business.industry Kallikrein chemotherapy target medicine.disease 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer research business Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | Pancreatic cancer is one of the deadliest cancers with very poor prognosis, and the five-year survival rate of the patients is less than 5% after diagnosis. Kallikrein-related peptidases (KLKs) belong to a serine protease family with 15 members that play important roles in cellular physiological behavior and diseases. The high expression level of KLK7 in pancreatic cancer tissues is considered to be a marker for the poor prognosis of this disease. In this work, we set out to investigate whether KLK7 could be a target for the treatment of pancreatic cancer. Short hairpin RNAs (shRNAs) were designed and constructed in lentivirus to knock down KLK7 in pancreatic cancer cell line PANC-1, and the real time cellular analysis (RTCA) was used to evaluate cell proliferation, migration and invasion abilities. Small molecules inhibiting KLK7 were discovered by computer-aided drug screening and used to inhibit PANC-1 cells. Our results confirmed that KLK7 is significantly up-regulated in pancreatic cancer tissue, and knocking down or inhibiting KLK7 efficiently inhibited the proliferation, migration and invasion of pancreatic cancer cells. This study suggested that KLK7 could be a potential chemotherapy target for treatment of pancreatic cancer, which would provide us a novel strategy for the treatment of this disease. |
| Databáze: | OpenAIRE |
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