Surface-layer protein produced by Lactobacillus crispatus JCM 2009 ameliorates lipopolysaccharide-induced inflammation through autophagy cross-talk with the NF-κB signaling pathway

Autor: Huifang Wang, Li Zhang, Shichen Xu, Qinpei Li, Rongrong Lu
Rok vydání: 2020
Předmět:
Lipopolysaccharides
Lipopolysaccharide
medicine.drug_class
Cell Survival
Inflammation
02 engineering and technology
Biochemistry
Anti-inflammatory
Nitric oxide
Microbiology
03 medical and health sciences
chemistry.chemical_compound
Mice
Phosphatidylinositol 3-Kinases
Lactobacillus acidophilus
Structural Biology
medicine
Autophagy
Animals
Prostaglandin E2
Lactobacillus crispatus
Molecular Biology
030304 developmental biology
Cell Nucleus
0303 health sciences
biology
Cell Death
Chemistry
TOR Serine-Threonine Kinases
NF-kappa B
Membrane Proteins
General Medicine
equipment and supplies
021001 nanoscience & nanotechnology
biology.organism_classification
Protein Transport
RAW 264.7 Cells
Cytokines
lipids (amino acids
peptides
and proteins)
medicine.symptom
Inflammation Mediators
0210 nano-technology
Proto-Oncogene Proteins c-akt
medicine.drug
Signal Transduction
Zdroj: International journal of biological macromolecules. 166
ISSN: 1879-0003
Popis: In recent years, studies on immunomodulation by surface-layer proteins (Slps) have mainly focused on Lactobacillus acidophilus, there is little information on Slp from L. crispatus and its intestinal immunomodulatory mechanisms in macrophages. In our study, the anti-inflammatory actions of Slp derived from L. crispatus JCM 2009 and its related molecular mechanisms were investigated. We initially found that incubation with Slp (5–10 μg/mL) for 4 h significantly inhibited nitric oxide (NO) and prostaglandin E2 (PGE2) production in LPS-stimulated RAW264.7 cells (P
Databáze: OpenAIRE
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