Enhanced production of matrix metalloproteinases and activation of matrix metalloproteinase 2 (gelatinase A) in human gastric carcinomas
| Autor: | Masayoshi Mai, Motoharu Seiki, Noboru Fujimoto, Yasunori Okada, Hidehiro Nomura |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Stromal cell Gelatinase A Matrix metalloproteinase Biology Immunoenzyme Techniques Stomach Neoplasms Zymogen medicine Carcinoma Humans Glycoproteins Enzyme Precursors Tissue Inhibitor of Metalloproteinase-2 Metalloendopeptidases Tissue Inhibitor of Metalloproteinases medicine.disease Immunohistochemistry Enzyme Activation Oncology Gelatinases Protein Biosynthesis Collagenase Cancer research Matrix Metalloproteinase 2 Interstitial collagenase Immunostaining medicine.drug |
| Zdroj: | International Journal of Cancer. 69:9-16 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/(sici)1097-0215(19960220)69:1<9::aid-ijc3>3.0.co;2-8 |
| Popis: | We examined the production and tissue localization of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in gastric carcinoma tissues. MMP-1 (tissue collagenase), MMP-9 (gelatinase B) and TIMP-2 were immunolocalized in carcinoma cells and MMP-2 (gelatinase A) on tumor cell membranes, whereas no or little immunostaining for MMP-3 (stromelysin-1) and TIMP-1 was seen in carcinoma cells. Stromal cells in carcinoma tissue were also positively stained for these MMPs and TIMPs. MMP-2 immunostaining was observed exclusively on advanced gastric carcinoma cells and correlated with vascular invasion by tumor cells. Sandwich enzyme immunoassays revealed enhanced production of MMP-1, MMP-2, MMP-3, MMP-9 and TIMP-1 by carcinoma tissues. Gelatinolytic activities were significantly higher in carcinoma samples than in normal controls. Using gelatin zymography, active forms of MMP-2 and MMP-9 were more frequently detected in carcinoma tissue, and the activation rate of the zymogen of MMP-2 (proMMP-2), but not that of proMMP-9, correlated well with degree of local invasion and lymphatic permeation. Our data indicate an enhanced production of 4 MMPs in gastric carcinoma tissue and suggest that activation of pro-MMP-2 may be a key step for spreading of gastric carcinoma cells. |
| Databáze: | OpenAIRE |
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