An infectious chimeric human immunodeficiency virus type 2 (HIV-2) expressing the HIV-1 principal neutralizing determinant
| Autor: | David Looney, Flossie Wong-Staal, R Talbott, M Mamounas |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Radioimmunoprecipitation Assay
T-Lymphocytes Immunology Molecular Sequence Data Biology V3 loop medicine.disease_cause Virus Replication Microbiology Polymerase Chain Reaction Virus Neutralization Cell Line Chimera (genetics) Virology medicine Tumor Cells Cultured Humans Cysteine DNA Primers Antiserum Base Sequence Chimera virus diseases Gene Products env Simian immunodeficiency virus Kinetics Viral replication Polyclonal antibodies Mutagenesis Insect Science HIV-2 biology.protein HIV-1 Simian Immunodeficiency Virus Research Article |
| Zdroj: | Journal of virology. 69(10) |
| ISSN: | 0022-538X |
| Popis: | The human immunodeficiency virus type 1 strain MN (HIV-1MN) principal neutralizing determinant (PND, V3 loop) was introduced into infectious molecular clones HIV-2KR and simian immunodeficiency virus mm239 (SIVmm239) by hybridization PCR, replacing the corresponding HIV-2 or SIV envelope cysteine loops with the HIV-1 coding sequence. The HIV-2 chimera (HIV-2KR-MNV3) was found to be capable of infecting a number of T-cell lymphoblastic cell lines as well as primary peripheral blood mononuclear cells. In contrast, the SIV chimera (SIV239MNV3) was not replication competent. Envelope produced by HIV-2KR-MNV3 but not the parental HIV-2KR was recognized by V3-specific and HIV-1-specific polyclonal antisera in radioimmunoprecipitation assays. HIV-2-specific antisera recognized both the chimeric and parental virus but not HIV-1MN. The chimeric HIV-2KR-MNV3 virus proved to be exquisitely susceptible to neutralization by HIV-1-specific and V3-specific antisera, suggesting the potential for use in animal models designed to test HIV-1 vaccine candidates which target the PND. |
| Databáze: | OpenAIRE |
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