Effect of berbamine on invasion and metastasis of human liver cancer SMMC-7721 cells and its possible mechanism
| Autor: | Ying Caoa, Jian-bo Cao, Li-li Liu, Binbin Yu, Jia-dong Yan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Berbamine Benzylisoquinolines Connexins Cx32 Metastasis chemistry.chemical_compound medicine Humans Gene silencing Neoplasm Invasiveness Pharmacology (medical) Neoplasm Metastasis Pre-Clinical Reports PI3K/AKT/mTOR pathway Cell Proliferation Pharmacology PI3K/AKT Dose-Response Relationship Drug biology urogenital system Akt/PKB signaling pathway Liver Neoplasms Cell migration biology.organism_classification medicine.disease migration and invasion berbamine Oncology chemistry Berberis Cancer research Phosphatidylinositol 3-Kinase Liver cancer Proto-Oncogene Proteins c-akt |
| Zdroj: | Anti-Cancer Drugs |
| ISSN: | 0959-4973 |
| Popis: | Berbamine is a bisbenzylisoquinoline alkaloid extracted from Berberis poiretii of Berberis of Berberidaceae. It has been reported that it can significantly inhibit the proliferation of a variety of malignant tumor cells, including liver cancer. However, the effect of berbamine on the invasion and metastasis of liver cancer has not been reported. The present study demonstrated that berbamine inhibited the migration and invasion of SMMC-7721 cells in a concentration-dependent manner and obviously increased the gap junction function and the expression of Cx32 in SMMC-7721 cells compared with control group. However, after silencing Cx32, berbamine had no significant effect on cell invasion and metastasis. Before silencing Cx32, the expression of PI3K and P-AKT were decreased after berbamine treated on SMMC-7721 cells for 24 h. After silencing Cx32, the expression of PI3K and P-AKT were increased in SMMC-7721 cells. The expression of PI3K and P-AKT had no significant effect after berbamine treated on SMMC-7721 cells for 24 h with silencing Cx32. In conclusion, the results of the present study suggest that berbamine could inhibit the SMMC-7721 cell migration and invasion, and its mechanism may be related to the regulation of PI3K/AKT signaling pathway by enhancing the expression of Cx32. |
| Databáze: | OpenAIRE |
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