Protein Binding of a Novel Platinum-Based Anticancer Agent BP-C1 Containing a Lignin-Derived Polymeric Ligand
| Autor: | Megan Westbury, Nikolay Solovyev, Sergey E. Pigarev, N. B. Ivanenko, Elena I. Fedoros |
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| Rok vydání: | 2021 |
| Předmět: |
Technology
QH301-705.5 QC1-999 lignin chemistry.chemical_element protein binding Plasma protein binding General Materials Science Equilibrium dialysis Biology (General) equilibrium dialysis inductively coupled plasma mass spectrometry QD1-999 Instrumentation Inductively coupled plasma mass spectrometry Fluid Flow and Transfer Processes Chromatography Physics Process Chemistry and Technology General Engineering Engineering (General). Civil engineering (General) Ligand (biochemistry) Computer Science Applications anticancer drugs Chemistry Membrane chemistry free platinum TA1-2040 Platinum Dialysis (biochemistry) Ex vivo |
| Zdroj: | Applied Sciences, Vol 11, Iss 11008, p 11008 (2021) |
| ISSN: | 2076-3417 |
| Popis: | Platinum (Pt) antineoplastic agents remain indispensable for the treatment of oncological disease. Pt-based drugs are mainly used in the therapy of ovarian cancer and non-small-cell lung carcinoma. A novel platinum-containing antineoplastic agent BP-C1 is a complex of diamminoplatinum with an oxygen-donor polymeric ligand of benzene-polycarboxylic acids, isolated from natural lignin. The aim of the study was to investigate ex vivo protein binding of BP-C1. Protein binding of BP-C1 was tested using equilibrium dialysis. Pooled blood plasma was used in the study. Control solutions contained the same dosages of BP-C1 in PBS (pH 7.2). Plasma and control solutions were submitted to equilibrium dialysis across a vertical 8 kDa cut-off membrane for 4 h at 37 °C under gentle shaking. Platinum was quantified in dialysis and retained fractions using inductively coupled plasma mass spectrometry after microwave digestion. The dialysis system was tested and validated; this showed no protein saturation with platinum. A medium degree of binding of platinum to macromolecular species of ca. 60% was observed. The study showed the maintenance of a high fraction of free BP-C1 in the bloodstream, facilitating its pharmacological activity. |
| Databáze: | OpenAIRE |
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