Wnt ligands regulate the asymmetric divisions of neuronal progenitors in C. elegans embryos
| Autor: | Pauline Mélénec, Guillaume Bordet, Sabrina Murgan, Vincent Bertrand, Shilpa Kaur, Pierre-François Lenne, Pierre Recouvreux |
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| Přispěvatelé: | Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Interneuron Wnt signaling pathway Embryo Biology Embryonic stem cell asymmetric division Wnt signaling neuron Cell biology 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure [SDV.BDD.EO]Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis medicine C. elegans Cholinergic polarity Neuron Progenitor cell Molecular Biology 030217 neurology & neurosurgery 030304 developmental biology Developmental Biology Progenitor |
| Zdroj: | Development (Cambridge, England) Development (Cambridge, England), Company of Biologists, 2020, 147 (7), pp.dev183186. ⟨10.1242/dev.183186⟩ Development (Cambridge, England), 2020, 147 (7), pp.dev183186. ⟨10.1242/dev.183186⟩ |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.183186⟩ |
| Popis: | International audience; Wnt/β-catenin signalling has been implicated in the terminal asymmetric divisions of neuronal progenitors in vertebrates and invertebrates. However, the role of Wnt ligands in this process remains poorly characterized. Here, we used the terminal divisions of the embryonic neuronal progenitors in C. elegans to characterize the role of Wnt ligands during this process, focusing on a lineage that produces the cholinergic interneuron AIY. We observed that, during interphase, the neuronal progenitor is elongated along the anteroposterior axis, then divides along its major axis, generating an anterior and a posterior daughter with different fates. Using time-controlled perturbations, we show that three Wnt ligands, which are transcribed at higher levels at the posterior of the embryo, regulate the orientation of the neuronal progenitor and its asymmetric division. We also identify a role for a Wnt receptor (MOM-5) and a cortical transducer APC (APR-1), which are, respectively, enriched at the posterior and anterior poles of the neuronal progenitor. Our study establishes a role for Wnt ligands in the regulation of the shape and terminal asymmetric divisions of neuronal progenitors, and identifies downstream components. |
| Databáze: | OpenAIRE |
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