Adiponectin Protects Against Hyperoxic Lung Injury and Vascular Leak
| Autor: | Christie A. Newland, Sainath R. Kotha, Sean M. Sliman, Jason P. Cruff, Travis O. Gurney, Shariq I. Sherwani, Carrie A. Schrader, Narasimham L. Parinandi, Ulysses J. Magalang, Rishi B. Patel |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Biophysics Adipokine Adipose tissue Mice Transgenic Lung injury Biology medicine.disease_cause Biochemistry Article Permeability Tight Junctions Lipid peroxidation Mice chemistry.chemical_compound Internal medicine medicine Animals Humans Lung Cytoskeleton Hyperoxia chemistry.chemical_classification Reactive oxygen species Endothelial Cells Lung Injury Cell Biology General Medicine Glutathione respiratory system Cell Hypoxia respiratory tract diseases Oxidative Stress Endocrinology chemistry Immunology Blood Vessels Cattle Female Adiponectin Lipid Peroxidation medicine.symptom Reactive Oxygen Species Oxidative stress |
| Zdroj: | Cell Biochemistry and Biophysics. 67:399-414 |
| ISSN: | 1559-0283 1085-9195 |
| Popis: | Adiponectin (Ad), an adipokine exclusively secreted by the adipose tissue, has emerged as a paracrine metabolic regulator as well as a protectant against oxidative stress. Pharmacological approaches of protecting against clinical hyperoxic lung injury during oxygen therapy/treatment are limited. We have previously reported that Ad inhibits the NADPH oxidase-catalyzed formation of superoxide from molecular oxygen in human neutrophils. Based on this premise, we conducted studies to determine whether (i) exogenous Ad would protect against the hyperoxia-induced barrier dysfunction in the lung endothelial cells (ECs) in vitro, and (ii) endogenously synthesized Ad would protect against hyperoxic lung injury in wild-type (WT) and Ad-overexpressing transgenic (AdTg) mice in vivo. The results demonstrated that exogenous Ad protected against the hyperoxia-induced oxidative stress, loss of glutathione (GSH), cytoskeletal reorganization, barrier dysfunction, and leak in the lung ECs in vitro. Furthermore, the hyperoxia-induced lung injury, vascular leak, and lipid peroxidation were significantly attenuated in AdTg mice in vivo. Also, AdTg mice exhibited elevated levels of total thiols and GSH in the lungs as compared with WT mice. For the first time, our studies demonstrated that Ad protected against the hyperoxia-induced lung damage apparently through attenuation of oxidative stress and modulation of thiol-redox status. |
| Databáze: | OpenAIRE |
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