The mechanisms for regulating absorption of Fe bis-glycine chelate and Fe-ascorbate in caco-2 cells are similar
| Autor: | Miguel Arredondo, Manuel Olivares, Marco T. Núñez, Dora Inés Mazariegos, Fernando Pizarro |
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| Rok vydání: | 2004 |
| Předmět: |
Absorption (pharmacology)
Analysis of Variance Nutrition and Dietetics Chemistry Enterocyte Iron Inorganic chemistry Glycine Medicine (miscellaneous) Biological Availability Ascorbic Acid Ascorbic acid Models Biological Ferrous Compounds Bioavailability Absorption medicine.anatomical_structure Caco-2 medicine Humans Chelation Caco-2 Cells Cells Cultured |
| Zdroj: | The Journal of nutrition. 134(2) |
| ISSN: | 0022-3166 |
| Popis: | Inorganic iron (Fe) absorption from the diet is controlled mainly in the intestinal tract where apical Fe uptake is inversely related to the Fe content in the enterocyte. Iron bis-glycine chelate is an iron compound that may be absorbed by a mechanism different from the regulated nonheme Fe pathway. Because Fe bis-glycine chelate is used increasingly as an Fe fortificant in foods, the critical question is whether this compound is a safe Fe supplement. We compared apical Fe uptake and transepithelial transport offered either as (59)Fe bis-glycine chelate or a (59)Fe-ascorbate (Fe-AA) complex in Caco-2 cells, as a model of human intestinal epithelia, grown in different Fe concentrations in the media (0.5, 5 and 20 micro mol/L Fe). Apical Fe uptake from (59)Fe-AA and (59)Fe bis-glycine chelate did not differ nor did transepithelial transport rates. The rate of (59)Fe uptake decreased with increasing intracellular Fe concentration (P < 0.001), an indication of a common absorption regulatory mechanism. We also evaluated the effect of an excess of Fe (100 micro mol/L) provided as Fe bis-glycine chelate or Fe-AA on the incorporation of 1 micro mol/L (55)Fe-AA into Fe-replete Caco-2 cells. The inhibition of Fe bis-glycine chelate on the absorption of the extrinsic tag of (55)Fe-AA (87.5%) did not differ from that of Fe added as Fe-AA (86.8%). These results suggest that Fe derived from Fe bis-glycine chelate and Fe-AA have similar regulatory absorption mechanisms. |
| Databáze: | OpenAIRE |
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