Multi-parameter comparison of a standardized mixed meal tolerance test in healthy and type 2 diabetic subjects: the PhenFlex challenge

Autor: Annette Stafleu, G.C.M. Bakker, Jacobus Burggraaf, Ben van Ommen, Linette Pellis, Alwine A. F. Kardinaal, Marjan van Erk, Ruud Boessen, Johanna H. M. Stroeve, Suzan Wopereis
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Endocrinology
Diabetes and Metabolism
Provocation test
Biomedical Innovation
Adipose tissue
Physiology
Type 2 diabetes
Muscle tissue
Time factor
0302 clinical medicine
Life
Response time
Nutritional challenge test
Liver tissue
Metabolic parameters
Metabolic stress
2. Zero hunger
Glucose metabolism
Kidney tissue
respiratory system
Crossover procedure
3. Good health
Diabetic patient
Retrospective study
Tissue distribution
Vascular tissue
Healthy Living
lcsh:Nutrition. Foods and food supply
Human
Adult
PhenFlex test
medicine.medical_specialty
lcsh:QH426-470
Clinical article
Metabolic health
Oral glucose tolerance test
030209 endocrinology & metabolism
lcsh:TX341-641
Clinical nutrition
Biology
Carbohydrate metabolism
Health status
03 medical and health sciences
Diet restriction
Metabolic regulation
Internal medicine
Biomarkers
Nutrition
Phenotypic flexibility
Genetics
medicine
Exploratory research
Human tissue
Oral antidiabetic agent
Aged
Research
Gold standard (test)
medicine.disease
Standardization
Intestine tissue
respiratory tract diseases
lcsh:Genetics
Glucose
MSB - Microbiology and Systems Biology
030104 developmental biology
Endocrinology
Human cell
Pancreas tissue
Non insulin dependent diabetes mellitus
ELSS - Earth
Life and Social Sciences
Controlled study
Homeostasis
Zdroj: Genes & Nutrition, Vol 12, Iss 1, Pp 1-14 (2017)
Genes & nutrition, 12:21
Genes & Nutrition
Genes & Nutrition, 21, 12
ISSN: 1865-3499
1555-8932
DOI: 10.1186/s12263-017-0570-6
Popis: Background A key feature of metabolic health is the ability to adapt upon dietary perturbations. Recently, it was shown that metabolic challenge tests in combination with the new generation biomarkers allow the simultaneous quantification of major metabolic health processes. Currently, applied challenge tests are largely non-standardized. A systematic review defined an optimal nutritional challenge test, the “PhenFlex test” (PFT). This study aimed to prove that PFT modulates all relevant processes governing metabolic health thereby allowing to distinguish subjects with different metabolic health status. Therefore, 20 healthy and 20 type 2 diabetic (T2D) male subjects were challenged both by PFT and oral glucose tolerance test (OGTT). During the 8-h response time course, 132 parameters were quantified that report on 26 metabolic processes distributed over 7 organs (gut, liver, adipose, pancreas, vasculature, muscle, kidney) and systemic stress. Results In healthy subjects, 110 of the 132 parameters showed a time course response. Patients with T2D showed 18 parameters to be significantly different after overnight fasting compared to healthy subjects, while 58 parameters were different in the post-challenge time course after the PFT. This demonstrates the added value of PFT in distinguishing subjects with different health status. The OGTT and PFT response was highly comparable for glucose metabolism as identical amounts of glucose were present in both challenge tests. Yet the PFT reports on additional processes, including vasculature, systemic stress, and metabolic flexibility. Conclusion The PFT enables the quantification of all relevant metabolic processes involved in maintaining or regaining homeostasis of metabolic health. Studying both healthy subjects and subjects with impaired metabolic health showed that the PFT revealed new processes laying underneath health. This study provides the first evidence towards adopting the PFT as gold standard in nutrition research. Electronic supplementary material The online version of this article (10.1186/s12263-017-0570-6) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
Externí odkaz: