Exercise performed during pregnancy positively modulates liver metabolism and promotes mitochondrial biogenesis of female offspring in a rat model of diet-induced gestational diabetes
| Autor: | Jelena Stevanović-Silva, Jorge Beleza, Pedro Coxito, Hugo Rocha, Tiago Bordeira Gaspar, Fátima Gärtner, Rossana Correia, Rui Fernandes, Paulo J. Oliveira, António Ascensão, José Magalhães |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Fetal Programming
Sucrose Organelle Biogenesis Gestational Exercise Peroxisome Proliferator-Activated Receptors Diet High-Fat Lipids Rats Doenças Genéticas Rats Sprague-Dawley Diabetes Gestational MicroRNAs Physical Exercise Liver Pregnancy Molecular Medicine Animals Humans Female RNA Messenger Molecular Biology |
| Popis: | Gestational diabetes mellitus (GDM) is associated with a high-risk for metabolic complications in offspring. However, exercise is recognized as a non-pharmacological strategy against metabolic disorders and is recommended in GDM treatment. This study aimed to investigate whether gestational exercise (GE) could modulate maternal high-fat high-sucrose (HFHS) diet-related hepatic metabolic and mitochondrial outcomes in female offspring of mothers with HFHS-induced GDM. Female Sprague-Dawley rats were fed with control or HFHS diet and kept sedentary or submitted to GE. Their female offspring were fed with control diet and kept sedentary. Hepatic lipid accumulation, lipid metabolism regulators, mitochondrial biogenesis and dynamics markers, and microRNAs associated to the regulation of these markers were evaluated. Female offspring of GDM mothers showed increased body weight at early age, whereas GE prevented this effect of maternal HFHS-feeding and reduced hepatic lipid accumulation. GE stimulated hepatic mRNA transcription and protein expression of mitochondrial biogenesis markers (peroxisome proliferator-activated receptor-gamma co-activator-1alpha and mitochondrial transcription factor A) and mRNA transcription of mitochondrial dynamics markers (mitofusin-1, mitofusin-2, and dynamin-related protein-1) that were altered by maternal GDM, while mitochondrial dynamics markers protein expression was not affected by maternal diet/GE except for optic atrophy-1. MicroRNAs associated with these processes (miR-122, miR-34a, miR-130b, miR-494), and the expression of auto/mitophagy- and apoptosis-related proteins were not substantially influenced by altered intrauterine environment. Our findings suggest that GE is an important regulator of the intrauterine environment positively affecting liver metabolism and promoting liver mitochondrial biogenesis in female offspring despite eventual effects of maternal HFHS-feeding and related GDM. Highlights: Maternal lifestyle can affect hepatic metabolic status of their female offspring; Female offspring hepatic metabolism is affected by maternal high-fat/sucrose diet; Gestational exercise mitigated maternal diet-related harmful outcomes on offspring liver; Gestational exercise is an important regulator of offspring mitochondrial biogenesis and dynamics; Exercise programs should be encouraged in clinical counseling during pregnancy. This work was supported by the EU's Horizon 2020 Research and Innovation program under the Marie Skłodowska-Curie Actions (No.722619, FOIE GRAS; No.734719, mtFOIE GRAS) and by the Por tuguese Foundation for Science and Technology (FCT) (FCT/UID/DTP/00617/2020-base; POCI-01-0145-FEDER-016690-PTDC/DTP-DES/7087/2014; POCI-01-0145-FEDER-016657-PTDC/DTP-DES/1082/2014), to JB (SFRH/BD/129645/2017). info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|