The diagnostic value of serological studies in pediatric patients with acute Mycoplasma pneumoniae infection
| Autor: | Nan-Chang Chiu, Lung Chang, Lih-Ju Lin, Ching-Ying Huang, Yen-Hsin Kung, Fu-Chieh Chang, Daniel Tsung-Ning Huang, Wai-Tim Jim, Hsin Chi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Male Mycoplasma pneumoniae Adolescent 030106 microbiology Taiwan lcsh:QR1-502 medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Immunoglobulin G lcsh:Microbiology Serology Throat culture 03 medical and health sciences 0302 clinical medicine Pneumonia Mycoplasma Immunology and Allergy Medicine Humans Serologic Tests 030212 general & internal medicine Prospective Studies Child Respiratory Tract Infections General Immunology and Microbiology Respiratory tract infections medicine.diagnostic_test biology business.industry Diagnostic Tests Routine Infant General Medicine Mycoplasma Antibodies Bacterial Immunoglobulin A Infectious Diseases Immunoglobulin M Child Preschool Immunology biology.protein Population study Female business |
| Zdroj: | Journal of Microbiology, Immunology and Infection, Vol 53, Iss 2, Pp 351-356 (2020) |
| ISSN: | 1684-1182 |
| Popis: | Background: Mycoplasma pneumoniae is a common pathogen of respiratory tract infections in pediatric patients. Serological studies are traditional methods for the diagnosis. However, early diagnosis of M. pneumoniae infections remains problematic. We investigate the value of early serum immunoglobulin A (IgA), in addition to immunoglobulin G (IgG), and immunoglobulin M (IgM) levels, in children infected with M. pneumoniae. Methods: From August 2016 to February 2017, we enrolled pediatric patients based on both clinical symptoms and chest x-ray, and confirmed by positive throat culture for M. pneumoniae. Serum titers of M. pneumoniae IgM, IgG, and IgA during the acute phase were checked. All respiratory samples were further analyzed by polymerase chain reaction (PCR). Diagnostic values of different tests were evaluated. Results: Fifty-six patients fulfilled the diagnostic criteria, with a median age of 4.84 years. Most of them (89.3%) were enrolled within 7 days of disease onset. PCR was positive in 71.4% of the study population. Early IgG samples were of limited value in diagnosing M. pneumoniae infection, of which 89.3% showed a negative result. Positive rates of early serum IgA and IgM were 48.2% and 46.4%, respectively. In combination with IgA and/or IgM, the sensitivity increased to 71.4% during their early clinical course. Conclusions: In the pediatric population, combined serological tests of M. pneumoniae IgA and IgM, offer an accurate method of early diagnosis comparable to that of PCR, and can be an alternative choice for prompt detection of mycoplasma infections when PCR and culture are not available. Keywords: Mycoplasma pneumoniae, Children, Diagnosis, Culture, Serology |
| Databáze: | OpenAIRE |
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