Hungatella effluvii gen. nov., sp. nov., an obligately anaerobic bacterium isolated from an effluent treatment plant, and reclassification of Clostridium hathewayi as Hungatella hathewayi gen. nov., comb. nov
| Autor: | Sukhpreet Kaur, K. Suresh, Mir Yawar, Pawan Kumar |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Gram-Positive Endospore-Forming Rods Molecular Sequence Data Microbiology Aesculin chemistry.chemical_compound Bioreactors Clostridium RNA Ribosomal 16S Gene cluster Phylogeny Ecology Evolution Behavior and Systematics Clostridium hathewayi Base Composition biology Strain (chemistry) Probiotics Fatty Acids Sequence Analysis DNA General Medicine Ribosomal RNA biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques Type species chemistry Fermentation |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 64:710-718 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.056986-0 |
| Popis: | A Gram-stain-positive, rod-shaped, spore-forming and strictly anaerobic bacterium, designated UB-B.2T, was isolated from an industrial effluent anaerobic digester sample. It grew optimally at 30 °C and pH 7.0. Comparative analysis of the 16S rRNA gene sequence confirmed that strain UB-B.2T was closely related to Clostridium hathewayi DSM 13479T (97.84 % similarity), a member of rRNA gene cluster XIVa of the genus Clostridium , and formed a coherent cluster with other related members of the Blautia ( Clostridium ) coccoides rRNA group in phylogenetic analyses. The end products of glucose fermentation by strain UB-B.2T were acetate and propionate. The G+C content of the DNA was 51.4 mol%. Although strain UB-B.2T showed 97.8 % 16S rRNA gene sequence identity to the type strain of C. hathewayi , it exhibited only 38.4 % relatedness at the whole-genome level. It also showed differences from its closest phylogenetic relative, C. hathewayi DSM 13479T, in phenotypic characteristics such as hydrolysis of aesculin, starch and urea and fermentation end products. Both strains showed phenotypic differences from the members of rRNA gene cluster XIVa of the genus Clostridium . Based on these differences, C. hathewayi DSM 13479T and strain UB-B.2T were identified as representatives of a new genus of the family Clostridiaceae . Thus, we propose the reclassification of Clostridium hathewayi as Hungatella hathewayi gen. nov., comb. nov., the type species of the new genus (type strain DSM 13479T = CCUG 43506T = MTCC 10951T). Strain UB-B.2T ( = MTCC 11101T = DSM 24995T) is assigned to the novel species Hungatella effluvii gen. nov., sp. nov as the type strain. |
| Databáze: | OpenAIRE |
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