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Additional file 7: Supplementary Figure 7. BSP expression after different treatments during 48 h in MCF-7. BSP was evidenced by immunofluorescence and is expressed in the cytoplasm. Estrogens (E) and CDCA (CDCA) induced an increase of BSP expression compared to the control (C). 4-hydroxytamoxifen (T), fulvestrant (F), LCA (L) and Z-guggulsterone (G) caused no variation in BSP expression compared to the control (C). 4-hydroxytamoxifen and fulvestrant in combined with estrogens or CDCA (E + T, E + F, CDCA+T, CDCA+F) caused a decrease of BSP expression compared to estrogens (E) or CDCA (CDCA). LCA and Z-guggulsterone in combined with CDCA (CDCA+L, CDCA+G) caused a decrease in BSP expression compared to CDCA (CDCA). Scale bars = 100 μm. |
