Neuroblastoma cells produce transforming growth factors during exponential growth in a defined hormone-free medium
| Autor: | T. M. J. Van Oostwaard, S.W. de Laat, G J Todaro, E.J.J. van Zoelen, D R Twardzik, P. T. Van Der Saag |
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| Rok vydání: | 1984 |
| Předmět: |
DNA Replication
Peptide Biosynthesis Cell division medicine.medical_treatment Receptors Cell Surface Biology Cell Line Mice Neuroblastoma Epidermal growth factor medicine Animals Doubling time Receptor Autocrine signalling Cells Cultured Multidisciplinary Epidermal Growth Factor Growth factor Molecular biology Culture Media Neoplasm Proteins ErbB Receptors Kinetics Cell Transformation Neoplastic Cell culture Transforming Growth Factors Peptides Cell Division Research Article Transforming growth factor |
| Zdroj: | Proceedings of the National Academy of Sciences. 81:4085-4089 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Mouse neuroblastoma Neuro-2A cells have been cultured in a chemically defined serum-free medium consisting of a 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F-12 medium, supplemented with 30 nM selenite and 10 micrograms of transferrin per ml. In this medium, which does not contain any externally added polypeptide growth factor, cells proliferate rapidly with a doubling time of approximately equal to 10 hr. During exponential growth in this serum-free medium, Neuro-2A cells secrete a 15- to 20-kDa transforming growth factor with strong mitogenic action and the ability to induce anchorage-independent growth on nontransformed cells. This neuroblastoma-derived transforming growth factor (ND-TGF) is acid and heat stable but is sensitive to treatment with trypsin or dithiothreitol. However, it does not compete with epidermal growth factor (EGF) for receptor binding and does not require EGF receptors for its mitogenic activity. Experiments on the effects of EGF on ND-TGF-induced soft agar growth of normal rat kidney cells indicate that Neuro-2A cells secrete an EGF-potentiated TGF in addition to ND-TGF. It is suggested that Neuro-2A cells can proliferate in the absence of externally added growth factors as a result of autocrine production of polypeptide growth factors. |
| Databáze: | OpenAIRE |
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