Urotensin-II receptor is over-expressed in colon cancer cell lines and in colon carcinoma in humans

Autor:	Paola Stiuso, Michele Caraglia, Paolo Grieco, Carmela Loguercio, Antonietta Gerarda Gravina, F. P. D'armiento, Silvia Zappavigna, Alessandro Federico, Ettore Novellino, Marco Romano, Monica Marra, Giovanni Vitale, Concetta Tuccillo, Amalia Luce
Přispěvatelé:	Federico, Alessandro, Zappavigna, Silvia, Romano, Marco, Grieco, Paolo, Luce, Amalia, Marra, Monica, Gravina, Antonietta Gerarda, Stiuso, Paola, D'Armiento, Francesco Paolo, Vitale, Giovanni, Tuccillo, Concetta, Novellino, Ettore, Loguercio, Carmela, Caraglia, Michele, Zappavigna, S, Grieco, P, Luce, A, Marra, M, Gravina, Ag, D’Armiento, Fp, Vitale, G, Tuccillo, C, Novellino, E, Loguercio, Carmelina
Rok vydání:	2014
Předmět:	Male Untranslated region Colorectal cancer Clinical Biochemistry Biochemistry Receptors G-Protein-Coupled Small hairpin RNA chemistry.chemical_compound Peptide Fragment Cell Movement Receptor Aged 80 and over Colonic Neoplasm Gene knockdown Medicine (all) General Medicine Middle Aged Colon cancer Urotensin-II receptor Gene Knockdown Techniques Colonic Neoplasms Female Growth inhibition HT29 Cells Human Adenoma Colon Urotensins Colonic Polyps Adenocarcinoma Biology Cell Line Tumor medicine Humans Neoplasm Invasiveness RNA Messenger Aged Cell Proliferation Neoplasm Invasivene Messenger RNA medicine.disease Molecular biology Peptide Fragments digestive system diseases Colonic Polyp HT29 Cell chemistry Urotensin-II Gene Knockdown Technique Urotensin
Zdroj:	European Journal of Clinical Investigation. 44:285-294
ISSN:	0014-2972
Popis:	BACKGROUND: Urotensin (U)-II receptor (UTR) has been previously reported to be over-expressed in a number of tumours. Whether UTR-related pathway plays a role in colon carcinogenesis is unknown. METHODS: We evaluated UTR protein and mRNA expression in human epithelial colon cancer cell lines and in normal colon tissue, adenomatous polyps and colon cancer. U-II protein expression was assessed in cancer cell lines. Moreover, we evaluated the effects of U-II(4-11) (an UTR agonist), antagonists and knockdown of UTR protein expression through a specific shRNA, on proliferation, invasion and motility of human colon cancer cells. RESULTS: Cancer cell lines expressed U-II protein and UTR protein and mRNA. By immunohistochemistry, UTR was expressed in 5-30% of epithelial cells in 45 normal controls, in 30-48% in 21 adenomatous polyps and in 65-90% in 48 colon adenocarcinomas. UTR mRNA expression was increased by threefold in adenomatous polyps and eightfold in colon cancer, compared with normal colon. U-II(4-11) induced a 20-40% increase in cell growth while the blockade of the receptor with specific antagonists caused growth inhibition of 20-40%. Moreover, the knock down of UTR with a shRNA or the inhibition of UTR with the antagonist urantide induced an approximately 50% inhibition of both motility and invasion. CONCLUSIONS: UTR appears to be involved in the regulation of colon cancer cell invasion and motility. These data suggest that UTR-related pathway may play a role in colon carcinogenesis and that UTR may function as a target for therapeutic intervention in colon cancer.
Databáze:	OpenAIRE
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