Magnetic nanoparticles coated with carboxylate-terminated carbosilane dendrons as a reusable and green approach to extract/purify proteins
| Autor: | Prados Nieto, Isabel María, Sánchez-Nieves Fernández, Javier, Mata de la Mata, Francisco Javier de la, Marina Alegre, María Luisa, García López, María Concepción |
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| Přispěvatelé: | Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química, Universidad de Alcalá. Departamento de Química Orgánica y Química Inorgánica |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | e_Buah Biblioteca Digital Universidad de Alcalá Institut de Recerca i Tecnologia Agroalimentàries (IRTA) Analytical and Bioanalytical Chemistry |
| Popis: | Extraction/purification of proteins, at both analytical and industrial levels, is a limiting step that usually requires the use of organic solvents and involves tedious work and a high cost. This work proposes a more sustainable alternative based on the use of magnetic nanoparticles (MNPs) coated with carboxylate-terminated carbosilane dendrons. MNPs coated with first- and second-generation carbosilane dendrons and bare MNPs were employed for the extraction of proteins with different molecular weights and charges. Interaction of proteins with MNPs significantly varied with the pH, the protein, and the dendron generation (different sizes and number of charges in the periphery). Optimal dendron:protein molar ratios and suitable conditions for disrupting interactions after protein extraction were also researched. Second-generation dendron-coated MNPs showed 100% retention capability for all proteins when using acidic conditions. They were reused without losing magnetism or interaction capacity after a disruption of protein-dendron interactions with 0.2% SDS at 100 °C for 10 min. The capacity of dendron-coated MNPs was successfully applied to the recovery/purification of proteins from two food by-products, olive seeds and cheese whey. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00216-021-03794-7. |
| Databáze: | OpenAIRE |
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