Properties of potyvirus RNAs: turnip mosaic, tobacco etch, and maiza dwarf mosaic viruses
| Autor: | John H. Hill, Helen I. Benner |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Hot Temperature
Analytical chemistry Biology Nucleic Acid Denaturation Zea mays Plant Viruses Mosaic Viruses Virology Formaldehyde Botany Tobacco Centrifugation Density Gradient Denaturation (biochemistry) Polyacrylamide gel electrophoresis Gel electrophoresis Mosaic virus Hyperchromicity Potyvirus biology.organism_classification Sedimentation coefficient Molecular Weight Electrophoresis Plants Toxic Nucleic Acid Conformation RNA Viral Electrophoresis Polyacrylamide Gel |
| Zdroj: | Virology. 75(2) |
| ISSN: | 0042-6822 |
| Popis: | Ribonucleic acids (RNAs) of turnip mosaic (TurMV), tobacco etch (TEV), and maize dwarf mosaic viruses (MDMV) have molecular weights of 3.31 × 106, 3.14 × 106, and 2.89 × 106 determined by polyacrylamide gel electrophoresis, and of 3.07 × 106, 3.33 × 106, and 2.94 × 106 determined by sedimentation analysis in linear log sucrose density gradients. These correspond to S values of 39.0, 40.6 and 38.2. When denatured by formaldehyde, molecular weights are TurMV, 3.07 × 106; TEV, 3.04 × 106; and MDMV, 2.90 × 106 determined by gel electrophoresis. Sedimentation coefficients after denaturation are TurMV, 24.7 S; TEV, 24.9 S; and MDMV, 24.2 S, corresponding to molecular weights of 3.16 × 106, 3.32 × 106, and 3.06 × 106 for TurMV, TEV, and MDMV, respectively. The reduction in electrophoretic mobility and sedimentation coefficient after denaturation, along with hyperchromicity and Tm measurements, suggests that all three virus RNAs have a similar degree of secondary structure. |
| Databáze: | OpenAIRE |
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