Creatine Monohydrate Supplementation Increases White Adipose Tissue Mitochondrial Markers in Male and Female Rats in a Depot Specific Manner

Autor: Chantal R. Ryan, Jensen E. Murphy, Rebecca E. K. MacPherson, Michael S. Finch, Tyler Dunham, Brian D. Roy
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Male
medicine.medical_specialty
Adipose Tissue
White
Adipose tissue
White adipose tissue
Biology
Creatine
Article
Electron Transport Complex IV
Rats
Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Sex Factors
Adipose Tissue
Brown
Internal medicine
Adipocyte
Brown adipose tissue
medicine
Animals
Pyruvate Dehydrogenase (Lipoamide)
TX341-641
Uncoupling Protein 1
Nutrition and Dietetics
Dose-Response Relationship
Drug
Nutrition. Foods and food supply
Cytochromes c
thermogenesis
Thermogenin
adipose tissue
mitochondria
030104 developmental biology
medicine.anatomical_structure
Endocrinology
creatine
chemistry
Dietary Supplements
Female
Creatine Monohydrate
Thermogenesis
030217 neurology & neurosurgery
Food Science
Body Temperature Regulation
Zdroj: Nutrients
Volume 13
Issue 7
Nutrients, Vol 13, Iss 2406, p 2406 (2021)
ISSN: 2072-6643
DOI: 10.3390/nu13072406
Popis: White adipose tissue (WAT) is a dynamic endocrine organ that can play a significant role in thermoregulation. WAT has the capacity to adopt structural and functional characteristics of the more metabolically active brown adipose tissue (BAT) and contribute to non-shivering thermogenesis under specific stimuli. Non-shivering thermogenesis was previously thought to be uncoupling protein 1 (UCP1)-dependent however, recent evidence suggests that UCP1-independent mechanisms of thermogenesis exist. Namely, futile creatine cycling has been identified as a contributor to WAT thermogenesis. The purpose of this study was to examine the efficacy of creatine supplementation to alter mitochondrial markers as well as adipocyte size and multilocularity in inguinal (iWAT), gonadal (gWAT), and BAT. Thirty-two male and female Sprague-Dawley rats were treated with varying doses (0 g/L, 2.5 g/L, 5 g/L, and 10 g/L) of creatine monohydrate for 8 weeks. We demonstrate that mitochondrial markers respond in a sex and depot specific manner. In iWAT, female rats displayed significant increases in COXIV, PDH-E1alpha, and cytochrome C protein content. Male rats exhibited gWAT specific increases in COXIV and PDH-E1alpha protein content. This study supports creatine supplementation as a potential method of UCP1-independant thermogenesis and highlights the importance of taking a sex-specific approach when examining the efficacy of browning therapeutics in future research.
Databáze: OpenAIRE
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