Carbon nanotubes toxicology and effects on metabolism and immunological modificationin vitroandin vivo
| Autor: | Alessandra Cucina, Alessandra Tiberia, S Bosco, R Masciangelo, Gabriela Mazzanti, G. De Bellis, G Deriu, A M Chiaretti, Stefano Bellucci, C. Balasubramanian, Chiaretti M, G A Carru, Sabina Mastrangelo, A. Di Sotto, N Porta, Federico Micciulla, F Le Foche |
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| Rok vydání: | 2008 |
| Předmět: |
Cell type
Cell growth Chemistry Nanotechnology Carbon nanotube Condensed Matter Physics In vitro law.invention cells injuries/surgery multi-walled carbon nanotubes nanomaterials nanoparticles prosthetic hernia repair tissues toxicity law In vivo Cell culture Drug delivery Cancer research General Materials Science Carcinogen |
| Zdroj: | Journal of Physics: Condensed Matter. 20:474203 |
| ISSN: | 1361-648X 0953-8984 |
| DOI: | 10.1088/0953-8984/20/47/474203 |
| Popis: | The aim of this research is focused on the biological effects of multi wall carbon nanotubes (MWCNTs) on three different human cell types, laboratory animals in vivo, and immunological effects. Large numbers of researchers are directly involved in the handling of nanostructured materials such as MWCNTs and nanoparticles. It is important to assess the potential health risks related to their daily exposure to carbon nanotubes. The administration of sterilized nanosamples has been performed on laboratory animals, in both acute and chronic administration, and the pathological effects on the parenchymal tissues have been investigated. We studied the serum immunological modifications after intraperitoneal administration of the MWCNTs. We did not observe any antigenic reaction; the screening of ANA, anti-ENA, anti-cardiolipin, C-ANCA and P-ANCA was negative. No quantitative modification of immunoglobulins was observed, hence no modification of humoral immunity was documented. We also studied the effects of MWCNTs on the proliferation of three different cell types. MCF-7 showed a significant inhibition of proliferation for all conditions studied, whereas hSMCs demonstrated a reduction of cell growth only for the highest MWCNTs concentrations after 72 h. Also, no growth modification was observed in the Caco-2 cell line. We observed that a low quantity of MWCNTs does not provoke any inflammatory reaction. However, for future medical applications, it is important to realize prosthesis based on MWCNTs, through studying the corresponding implantation effects. Moreover, it has to be emphasized that this investigation does not address, at the moment, the carcinogenicity of MWCNTs, which requires a detailed follow-up investigation on the specific topic. In view of the subsequent and more extensive use of MWCNTs, especially in applications where carbon nanotubes are injected into the human body for drug delivery, as a contrast agent carrying entities for MRI, or as the basic material of a new prosthesis generation, more extended tests and experiments are necessary. |
| Databáze: | OpenAIRE |
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