New flow injection method for quality control of dietary supplements containing l-carnitine using extraction mediated by sodium taurodeoxycholate coacervate coupled to molecular fluorescence
| Autor: | Soledad Cerutti, Liliana Patricia Fernandez, Andrea C. Isaguirre, Gimena Acosta |
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| Rok vydání: | 2016 |
| Předmět: |
Analyte
PHARMACEUTICAL QUALITY CONTROL 02 engineering and technology Chloroformate 01 natural sciences Analytical Chemistry chemistry.chemical_compound medicine Carnitine Derivatization Spectroscopy Flow injection analysis Detection limit FLUORESCENCE DETECTION Coacervate Chromatography Otras Ciencias Químicas 010401 analytical chemistry Extraction (chemistry) Ciencias Químicas 021001 nanoscience & nanotechnology L-CARNITINE 0104 chemical sciences chemistry FLOW INJECTION ANALYSIS BILE SALT COACERVATION 0210 nano-technology CIENCIAS NATURALES Y EXACTAS medicine.drug |
| Zdroj: | Microchemical Journal. 129:268-273 |
| ISSN: | 0026-265X |
| DOI: | 10.1016/j.microc.2016.06.025 |
| Popis: | Carnitine (CAR) is an essential component of the tissue of animals, higher plants and many microorganisms; it plays an important role during the metabolism of the human body. CAR transfers fatty acids to the place of burning-mitochondria and aids in the transformation of fats into energy and in this way supports overweight reduction and immediate physical performance, increases resistance from physical load and protects heart from overload. In this study, a new methodology for the determination of L-CAR by molecular fluorescence is described, after derivatization with a fluorescence active substance, 9-fluorenylmethyl chloroformate. An on-line coacervation process was used in order to isolate the analyte of the complex matrix. The developed methodology was successfully applied to the control of carnitine content in dietary supplements. The detection limit and quantification obtained were 2.4 × 10− 8 mol L− 1 and 7.30 × 10− 8 mol L− 1; respectively. Recoveries between 82 and 103% were obtained. At optimized working conditions, the sample throughput was 30 samples h− 1 (without considering the required time for L-CAR derivatization). Thus short analysis and very low running costs compared with the LC/MS conventional methodology can be cited as the main advantages of the on-line coacervation with fluorescence detection proposed methodology. Fil: Isaguirre, Andrea Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; Argentina Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; Argentina |
| Databáze: | OpenAIRE |
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