Assessment of mammary gland metabolism in the sow: I. Development of methods for the measurement of cellular metabolites in milk and colostrum
| Autor: | Peter E. Hartmann, Craig S. Atwood |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Swine Mammary gland chemistry.chemical_element Lactose Fructose Calcium Biology Sensitivity and Specificity Phosphates chemistry.chemical_compound Adenosine Triphosphate Mammary Glands Animal Internal medicine medicine Animals chemistry.chemical_classification Nucleotides Colostrum Galactose General Medicine Metabolism Milk Proteins Glucose Milk Whey Proteins Enzyme medicine.anatomical_structure Endocrinology chemistry Spectrophotometry Luminescent Measurements Female Animal Science and Zoology Food Science |
| Zdroj: | Journal of Dairy Research. 62:189-206 |
| ISSN: | 1469-7629 0022-0299 |
| DOI: | 10.1017/s0022029900030910 |
| Popis: | SUMMARYWe have modified deproteinization methods and a number of spectrophotometric and bioluminescent methods in order to measure the concentrations of cellular metabolites in small volumes (< 0·3 ml) of sows' colostrum and milk. For the majority of the assays, recoveries ranged from 92 to 105%. The binding of ATP and UTP to a calcium phosphate–citrate–caseinate complex in milk, and the decrease in ATP (92%/h) and UTP (18·1 %/h) concentrations during in vitro incubation of the whey fraction suggested that it was unlikely ATP and UTP (both < 1 µM) could exist free in sows' milk. The mean concentrations (range) of cellular metabolites in milk (6–11 d post partum) were: glucose, 669 µM (220–1367); glucose 6-phosphate, 63·0 µM (27·6–101·4); glucose 1-phosphate, 18·3 µM (13·1–24·8); UDPglucose, 296 µM (170–494); UDPgalactose, 635 µM (230–945); lactose, 162 mM (124–187); UDP, 105 µM (85–130); UMP, 1760 µM (1326–2587); inorganic phosphate, 135 mM (1·4–29·3); ATP, in vitro incubation of milk and of colostrum suggested that these postsecretory changes were probably due to the actions of enzymes present in mammary secretion. Collection of milk that has been most recently secreted (hind milk) will provide the most accurate indication of the intracellular concentrations of these metabolites. The concentrations of cellular metabolites in the milks from different species are compared. |
| Databáze: | OpenAIRE |
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