Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds
| Autor: | Dali Han, Kai Xu, Chuanyuan Chen, Qi Zhou, Tongtong Cui, Fei Teng, Wei Li, Jing Li, Qingqin Gao, Lu Guo, Guihai Feng |
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| Rok vydání: | 2019 |
| Předmět: |
lcsh:QH426-470
CRISPR-Associated Proteins Short Report Computational biology Biology Genome Genome engineering Mice 03 medical and health sciences Endonuclease 0302 clinical medicine Genome editing Mouse cells Animals Humans CRISPR crRNA lcsh:QH301-705.5 030304 developmental biology Gene Editing Trans-activating crRNA 0303 health sciences RNA Human cells Human genetics lcsh:Genetics lcsh:Biology (General) biology.protein CRISPR-Cas12a/Cpf1 CRISPR-Cas Systems 030217 neurology & neurosurgery |
| Zdroj: | Genome Biology, Vol 20, Iss 1, Pp 1-6 (2019) Genome Biology |
| ISSN: | 1474-760X |
| Popis: | CRISPR-Cas12a/Cpf1, a single RNA-guided endonuclease system, provides a promising tool for genome engineering. However, only three Cas12a orthologs have been employed for mammalian genome editing, and the editing efficiency as well as targeting coverage still requires improvements. Here, we harness six novel Cas12a orthologs for genome editing in human and mouse cells, some of which utilize simple protospacer adjacent motifs (PAMs) that remarkably increase the targeting range in the genomes. Moreover, we identify optimized CRISPR RNA (crRNA) scaffolds that can increase the genome editing efficiency of Cas12a. Electronic supplementary material The online version of this article (10.1186/s13059-019-1620-8) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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