Effect of in vitro and in vivo anakinra on cytokines production in Schnitzler syndrome
| Autor: | Virginie Dutoit-Lefevre, Emmanuel Faure, David Launay, Pierre-Yves Hatron, Myriam Labalette, C. Hauspie, Olivier Robineau, Eric Hachulla, Sylvain Dubucquoi, Vincent Sobanski |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Lipopolysaccharides
musculoskeletal diseases Anatomy and Physiology Urticaria Lipopolysaccharide Immunology Interleukin-1beta lcsh:Medicine Enzyme-Linked Immunosorbent Assay Inflammation Dermatology Real-Time Polymerase Chain Reaction Peripheral blood mononuclear cell Proinflammatory cytokine chemistry.chemical_compound Rheumatology In vivo Immune Physiology medicine Humans Schnitzler Syndrome lcsh:Science Biology DNA Primers Anakinra Multidisciplinary Interleukin-6 Tumor Necrosis Factor-alpha business.industry lcsh:R Immunity medicine.disease Interleukin 1 Receptor Antagonist Protein Schnitzler syndrome chemistry Immune System Leukocytes Mononuclear Cytokines Medicine Clinical Immunology Tumor necrosis factor alpha lcsh:Q medicine.symptom business Research Article medicine.drug |
| Zdroj: | PLoS ONE, Vol 8, Iss 3, p e59327 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | IL-1 receptor antagonist anakinra is usually highly efficient in Schnitzler syndrome (SS), a rare inflammatory condition associating urticaria, fever, and IgM monoclonal gammopathy. In this study, we aimed to assess lipopolysaccharide (LPS)-induced production of inflammatory cytokines by peripheral blood mononuclear cells (PBMCs) before and after 1 month of anakinra in patients with SS. LPS-induced production of IL-1β, IL-6 and TNFα was assessed by enzyme-linked immunosorbent assay with and without anakinra in vitro, and before and after 1 month (in vivo condition) of treatment in 2 patients with SS. Spontaneous production of IL-1β, IL-6 and TNF-α by PBMCs was similar in the patients and the healthy controls and was almost undetectable. Stimulation with LPS caused a higher release of cytokines from the patients than from the healthy controls. Before in vivo anakinra start, in vitro adjunction of anakinra reduced the high LPS-induced production of IL-1β and TNFα in both patients and of IL-6 in one patient. After 1 month of treatment with anakinra, while the patients had dramatically improved, there was also a marked reduction in LPS-induced cytokines production, which was almost normalized in one patient. This study shows an abnormal LPS-induced inflammatory cytokines production in SS, which can be decreased or even normalized by in vitro and in vivo anakinra. |
| Databáze: | OpenAIRE |
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