Identification of 16S rRNA mutations in Mycoplasma genitalium potentially associated with tetracycline resistance in vivo but not selected in vitro in M. genitalium and Chlamydia trachomatis
| Autor: | Arabella Touati, Olivia Peuchant, Bertille de Barbeyrac, Béatrice Berçot, S. Pereyre, Carla Balcon, Justine Garraud, Chloé Le Roy, Cécile Bébéar, Jean-Michel Molina |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) Tetracycline 030106 microbiology Chlamydia trachomatis Mycoplasma genitalium Biology urologic and male genital diseases medicine.disease_cause Microbiology Sexual and Gender Minorities 03 medical and health sciences 0302 clinical medicine Serial passage RNA Ribosomal 16S Prevalence medicine Humans Mycoplasma Infections Pharmacology (medical) 030212 general & internal medicine Homosexuality Male Pharmacology Doxycycline Tetracycline Resistance Mycoplasma 16S ribosomal RNA biology.organism_classification Infectious Diseases Mutation France Bacteria medicine.drug |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 76:1150-1154 |
| ISSN: | 1460-2091 0305-7453 |
| Popis: | Objectives Tetracyclines are widely used for the treatment of bacterial sexually transmitted infections (STIs) and recently have been used successfully for post-exposure prophylaxis of STIs in MSM. We investigated the in vitro and in vivo development of tetracycline resistance in Chlamydia trachomatis and Mycoplasma genitalium and evaluated 16S rRNA mutations associated with acquired resistance in other bacteria. Methods In vitro selection of resistant mutants of reference strains of C. trachomatis and M. genitalium was undertaken by serial passage in medium containing subinhibitory concentrations of tetracycline or doxycycline, respectively. The 16S rRNA gene of the two microorganisms was amplified and sequenced at different passages, as were those of 43 C. trachomatis- and 106 M. genitalium-positive specimens collected in France from 2013 to 2019. Results No tetracycline- or doxycycline-resistant strains of C. trachomatis and M. genitalium, respectively, were obtained after 30 serial passages. The tetracycline and doxycycline MICs were unchanged and analysis of the 16S rRNA gene, the molecular target of tetracyclines, of C. trachomatis and M. genitalium revealed no mutation. No mutation in the 16S rRNA gene was detected in C. trachomatis-positive specimens. However, six M. genitalium-positive specimens harboured a mutation potentially associated with tetracycline resistance without known prior tetracycline treatment for patients. Conclusions Tetracyclines did not select in vitro-resistant mutants of C. trachomatis or M. genitalium. However, 16S rRNA mutations either responsible for or associated with tetracycline resistance in other bacteria, including mycoplasma species, were identified in several M. genitalium-positive specimens. |
| Databáze: | OpenAIRE |
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