Flavonoid profiling of a traditional Chinese medicine formula of Huangqin Tang using high performance liquid chromatography
| Autor: | Li Chen, Zhong-Ming Zhou, Dunfang Wang, Weihao Wang, Yan-li Wang, Weipeng Yang, Hui-Hui Zhang, Yiwei Wang, Shuai-xing Zhuang, Tao Li |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
HPLC fingerprinting analysis LLOD linearity lower limit of detection Radix Scutellariae Carrageenin Flavonoid Active components LLOQ lower limit of quantification RSD relative standard deviation Traditional Chinese medicine Mass spectrometry High-performance liquid chromatography 03 medical and health sciences 0302 clinical medicine General Pharmacology Toxicology and Pharmaceutics Huangqin-Tang ICH International Conference on Harmonization chemistry.chemical_classification Flavonoids PCA principal component analysis Chromatography TCM traditional Chinese medicine S/N signal-to-noise ratio lcsh:RM1-950 CFDA China Food and Drug Administration HQT Huangqin Tang Paw edema 030104 developmental biology lcsh:Therapeutics. Pharmacology Multiple-component quantitative analysis chemistry Homogeneous HPLC high performance liquid chromatography 030220 oncology & carcinogenesis Original Article Antipyretic Anti-inflammatory LC–MSn liquid chromatography–tandem mass spectrometer |
| Zdroj: | Acta Pharmaceutica Sinica. B Acta Pharmaceutica Sinica B, Vol 6, Iss 2, Pp 148-157 (2016) |
| ISSN: | 2211-3835 |
| DOI: | 10.1016/j.apsb.2016.01.001 |
| Popis: | The quality control processes for herbal medicines have been problematic. Flavonoids are the major active components of Huangqin Tang (HQT, a traditional Chinese medicine formula). In this study, we used a combinative method approach consisting of chromatographic fingerprinting (high performance liquid chromatography; HPLC), quantitative methods and a pharmacodynamic evaluation model to analyze the flavonoids of HQT obtained from different sources. Ten batches of HQT were analyzed by the HPLC fingerprinting method and 26 common peaks were detected, of which 23 peaks corresponded with the chemical profile of HQT. In addition, 11 major compounds were identified by LC–MS analysis (liquid chromatography–tandem mass spectrometer; LC–MSn) and quantified by the HPLC quantitative method approach. The studied 10 batches of HQT were found to be homogeneous in their composition with a similarity between 0.990 and 1.000. The distribution of the 11 identified compounds was found to be very similar among the batches. Only slight pharmacodynamic differences were detected between the different batches, confirming the homogeneity of HQT. The results of this study prove that the combination of chromatographic fingerprinting and quantitative analysis can be readily used for comprehensive quality control of herbal medicines. Graphical abstract Flavonoids are the major active components of Huangqin Tang (HQT, a traditional Chinese medicine formula). A combinative method approach consisting of chromatographic fingerprinting (high performance liquid chromatography; HPLC), quantitative methods and a pharmacodynamic evaluation model was used to analyze the flavonoids of HQT obtained from different sources and proved to be useful for comprehensive quality control of herbal medicines. Only slight pharmacodynamic differences were detected between different batches, confirming the homogeneity of HQT. fx1 |
| Databáze: | OpenAIRE |
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