Impaired secondary oxidant deactivation capacity and enhanced oxidative stress in serum from alveld affected lambs
| Autor: | Anne Bee Hegge, Trond Schumacher, Hanne Hjorth Tønnesen, Olav M. Skulberg, Carl M M Laane, Ivar Mysterud, Jan Karlsen |
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| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Biophysics Protoporphyrins Sheep Diseases Endogeny medicine.disease_cause Blood serum Hemolysis Alveld chemistry.chemical_compound Internal medicine medicine Secondary oxidant deactivation Animals Radiology Nuclear Medicine and imaging Photosensitizer Photosensitivity Disorders chemistry.chemical_classification Reactive oxygen species Minerals Radiation Sheep Radiological and Ultrasound Technology Protoporphyrin IX Singlet Oxygen Singlet oxygen medicine.disease Oxidants Oxidative Stress Endocrinology chemistry Biochemistry Oxidative stress |
| Zdroj: | Hegge, A B, Mysterud, I, Karlsen, J, Skulberg, O M, Laane, C M M, Schumacher, T & Tønnesen, H H 2013, ' Impaired secondary oxidant deactivation capacity and enhanced oxidative stress in serum from alveld affected lambs ', Journal of Photochemistry and Photobiology, B: Biology, vol. 126, pp. 126-134 . https://doi.org/10.1016/j.jphotobiol.2013.07.005 |
| ISSN: | 1873-2682 |
| Popis: | Alveld is a hepatogenous photosensitivity disorder in lambs. The aim of the study was to investigate if alveld affected lambs had a reduced capacity to handle oxidative stress induced from either endogenous and/or exogenous photosensitizers. Serum samples from alveld lambs (n = 33) were compared to serum samples from control lambs (n = 31) and exposed to a controlled amount of singlet oxygen ( 1O 2). The sera from alveld lambs were found to have an impaired ability to deactivate reactive oxygen species (ROS) compared to control sera. A higher degree of initial hemolysis and a higher concentration of the exogenous photosensitizer phytoporphyrin (PP) were detected in alveld sera compared to the controls. The action spectrum for the formation of 1O 2 indicated that PP as well as the endogenous compound proto-porphyrin IX (PP IX) may act as in vivo photosensitizers. A relatively high level of iron was detected in pooled serum from alveld lambs that showed a high degree of hemolysis. It was concluded that alveld photosensitivity is likely to be initiated by a photodynamic reaction involving PP and possibly also PP IX followed by a light-independent reaction involving hemoglobin-related products and catalysis by the Fenton reaction. |
| Databáze: | OpenAIRE |
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