Extraction of small molecule from human plasma by prototyping 3D printed sorbent through extruded filament for LC-MS/MS analysis
| Autor: | Upadhyayula Suryanarayana Murty, Srikanth Ponneganti, Daya Raju Adye, Subham Banerjee, P. Radhakrishnanand, Roshan M. Borkar, Tushar Kanti Malakar |
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| Rok vydání: | 2021 |
| Předmět: |
Rapid prototyping
Vinyl alcohol Bioanalysis Sorbent Chromatography Fused deposition modeling business.industry Extraction (chemistry) 3D printing Biochemistry Analytical Chemistry law.invention Drug Liberation chemistry.chemical_compound chemistry Tandem Mass Spectrometry law Printing Three-Dimensional Humans Technology Pharmaceutical Environmental Chemistry Sample preparation business Spectroscopy Chromatography Liquid |
| Zdroj: | Analytica Chimica Acta. 1187:339142 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2021.339142 |
| Popis: | Analytical sample preparation techniques are regarded as crucial steps for analyzing compounds from different biological matrices. The development of new extraction techniques is a modern trend in the bioanalytical sciences. 3D printed techniques have emerged as a valuable technology for prototyping devices in customized shapes for a cost-effective way to advance analytical sample preparation techniques. The present study aims to fabricate customized filaments through the hot-melt extrusion (HME) technique followed by fused deposition modeling mediated 3D printing process for rapid prototyping of 3D printed sorbents to extract a sample from human plasma. Thus, we fabricated our own indigenous filament using poly (vinyl alcohol), Eudragit® RSPO, and tri-ethyl citrate through HME to prototype the fabricated filament into a 3D printed sorbent for the extraction of small molecules. The 3D sorbent was applied to extract hydrocortisone from human plasma and analyzed using a validated LC-MS/MS method. The extraction procedure was optimized, and the parameters influencing the sorbent extraction were systematically investigated. The extraction recovery of hydrocortisone was found to be82% at low, medium, and high quality control samples, with a relative standard deviation of2%. The intra-and inter-day precisions for hydrocortisone ranged from 1.0% to 12% and 2.0%-10.0%, respectively, whereas the intra-and inter-day accuracy for hydrocortisone ranged from 93.0% to 111.0% and 92.0% to 110.0%, respectively. The newly customizable size and shape of the 3D printed sorbent opens new possibilities for extracting small molecules from human plasma. |
| Databáze: | OpenAIRE |
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