Smart Hydrogel Particles: Biomarker Harvesting: One-Step Affinity Purification, Size Exclusion, and Protection against Degradation
| Autor: | Alessandra Luchini, David H. Geho, Barney Bishop, Duy Tran, Cassandra Xia, Robert L. Dufour, Clinton D. Jones, Virginia Espina, Alexis Patanarut, Weidong Zhou, Mark M. Ross, Alessandra Tessitore, Emanuel F. Petricoin, Lance A. Liotta |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Analyte
Chromatography Chemistry Hydrolysis Mechanical Engineering Size-exclusion chromatography Hydrogels Bioengineering General Chemistry Condensed Matter Physics Chromatography Affinity Article Affinity chromatography Self-healing hydrogels Chromatography Gel Degradation (geology) Particle General Materials Science Biomarkers Macromolecule |
| Zdroj: | Nano Letters. 8:350-361 |
| ISSN: | 1530-6992 1530-6984 |
| Popis: | Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|