Interacting climate change factors (CO2 and temperature cycles) effects on growth, secondary metabolite gene expression and phenotypic ochratoxin A production by Aspergillus carbonarius strains on a grape-based matrix
| Autor: | Giancarlo Perrone, Massimo Ferrara, Carol Verheecke-Vaessen, Esther Garcia-Cela, Antonia Gallo, Donato Magistà, Naresh Magan, Angel Medina, Carla Cervini |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Ecophysiology Ochratoxin A ecophysiology biosynthetic genes Biology Secondary metabolite 01 natural sciences 03 medical and health sciences chemistry.chemical_compound mycotoxins Gene expression Genetics medicine Food science Mycotoxin Ecology Evolution Behavior and Systematics 030304 developmental biology Regulator gene black aspergilli Wine 0303 health sciences Velvet carbon dioxide biology.organism_classification Infectious Diseases black aspergilli alternating temperatures carbon dioxide mycotoxins ecophysiology biosynthetic genes chemistry alternating temperatures 010606 plant biology & botany medicine.drug |
| Zdroj: | Fungal biology (Online) (2019). doi:10.1016/j.funbio.2019.11.001 info:cnr-pdr/source/autori:Carla Cervini, Carol Verheecke-Vaessen, Massimo Ferrara, Esther García-Cela, Donato Magistà, Angel Medina, Antonia Gallo, Naresh Magan, Giancarlo Perrone/titolo:Interacting climate change factors (CO2 and temperature cycles) effects on growth, secondary metabolite gene expression and phenotypic ochratoxin A production by Aspergillus carbonarius strains on a grape-based matrix./doi:10.1016%2Fj.funbio.2019.11.001/rivista:Fungal biology (Online)/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume |
| Popis: | Little is known on the impact that climate change (CC) may have on Aspergillus carbonarius and Ochratoxin A (OTA) contamination of grapes, especially in the Mediterranean region - a hot spot for the impact of CC scenarios with temperature expected to increase by +2-5°C and CO2 to double or triple (400 vs 800/1200 ppm). This study examined the effect of (i) current and increased temperature in the alternating 11.5h dark/12.5h light cycle (15-28°C vs 18-34°C), representative of the North Apulia area, South Italy and (ii) existing and predicted CO2 concentrations (400 vs 1000 ppm), on growth, expression of biosynthetic genes (AcOTApks, AcOTAnrps, AcOTAhal, AcOTAp450, AcOTAbZIP) and regulatory genes of Velvet complex (laeA/veA/velB, "velvet complex") involved in OTA biosynthesis and OTA phenotypic production by three strains of A. carbonarius. The experiments made on a grape-based matrix showed that elevated CO2 resulted in a general stimulation of growth and OTA production. These results were supported by the up-regulation of both structural and regulatory genes involved in the OTA biosynthesis in elevated CO2 condition. Our work has shown for the first time that elevated CO2 concentration in the Mediterranean region may result in an increased risk of OTA contamination in the wine production chain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|