Continuous imaging of plasmon rulers in live cells reveals early-stage caspase-3 activation at the single-molecule level
| Autor: | Daniel R. Hostetter, Young-wook Jun, Charles S. Craik, Sassan Sheikholeslami, Cheryl Tajon, A. Paul Alivisatos |
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| Rok vydání: | 2009 |
| Předmět: |
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Protein Conformation Metal Nanoparticles Molecular Probe Techniques Nanoparticle Apoptosis Nanotechnology Cell Line Humans Scattering Radiation Molecule Surface plasmon resonance Metal nanoparticles Plasmon chemistry.chemical_classification Multidisciplinary Caspase 3 Biomolecule Surface Plasmon Resonance Photobleaching Enzyme Activation Kinetics chemistry Molecular Probes Physical Sciences Gold Signal Transduction |
| Zdroj: | Proceedings of the National Academy of Sciences. 106:17735-17740 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0907367106 |
| Popis: | The use of plasmon coupling in metal nanoparticles has shown great potential for the optical characterization of many biological processes. Recently, we have demonstrated the use of “plasmon rulers” to observe conformational changes of single biomolecules in vitro. Plasmon rulers provide robust signals without photobleaching or blinking. Here, we show the first application of plasmon rulers to in vivo studies to observe very long trajectories of single biomolecules in live cells. We present a unique type of plasmon ruler comprised of peptide-linked gold nanoparticle satellites around a core particle, which was used as a probe to optically follow cell-signaling pathways in vivo at the single-molecule level. These “crown nanoparticle plasmon rulers” allowed us to continuously monitor trajectories of caspase-3 activity in live cells for over 2 h, providing sufficient time to observe early-stage caspase-3 activation, which was not possible by conventional ensemble analyses. |
| Databáze: | OpenAIRE |
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