Modulation of adhesion, spreading and cytoskeleton organization of 3T3 fibroblasts by sulfonic groups present on polymer surfaces
| Autor: | Hanna M. Kowalczyńska, Małgorzata Nowak-Wyrzykowska |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Integrin beta Chains
Cytoskeleton organization Integrin alpha4 Integrin 3T3 cells Mice Cell Movement Tubulin medicine Cell Adhesion Animals Actinin Cell adhesion Cytoskeleton Cell Size biology Sulfur Compounds Chemistry Antibodies Monoclonal Cell Biology General Medicine Adhesion 3T3 Cells Vinculin Fibroblasts Cell biology Fibronectins Fibronectin medicine.anatomical_structure Biophysics biology.protein Polystyrenes Integrin alpha5beta1 |
| Zdroj: | Cell biology international. 27(2) |
| ISSN: | 1065-6995 |
| Popis: | The early phase of 3T3 fibroblast interaction with sulfonated styrene copolymer surfaces, of two sulfonic group densities and thus of differing wettability, was studied. The sulfonic groups present on copolymer surfaces affected the behaviour of cells, i.e. they stimulated cell adhesion, activated cell spreading and influenced cytoskeleton reorganization. The relative number of adhering cells correlated, while the number of spreading cells inversely correlated, with the surface density of sulfonic groups. Cell shape and the pattern of distribution of F-actin, alpha-actinin and vinculin in the interacting cells also depend on the surface density of sulfonic groups. On surfaces of high sulfonic group density, highly polarized cells were observed with F-actin bundles. On surfaces of low sulfonic group density, the cells spread with a square-like morphology with F-actin organized in stress fibres. In contrast, the cells spread poorly on nonsulfonated surfaces and cell adhesion was unaffected by surface wettability. The contribution of alpha(5)beta(1), alpha(4), and beta(5)integrins to the cell interaction with fibronectin (FN) and vitronectin (VN) adsorbed from serum-containing medium on polymer surfaces was examined. Our results suggest that surface sulfonic groups influence the conformation of FN and VN adsorbed on polymer surfaces and, in turn, determine the integrins that are involved in cell adhesion. |
| Databáze: | OpenAIRE |
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