Atherosclerosis and Bone Loss in Humans–Results From Deceased Donors and From Patients Submitted to Carotid Endarterectomy
| Autor: | Diana Carmona-Fernandes, Sofia C. Barreira, Natacha Leonardo, Renata I. Casimiro, Alice M. Castro, Pedro Oliveira Santos, António N. Fernandes, Filipe Cortes-Figueiredo, Carolina A. Gonçalves, Rafael Cruz, Mariana L. Fernandes, Margarida Ivo, Luis M. Pedro, Helena Canhão, João Eurico Fonseca, Maria José Santos |
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| Přispěvatelé: | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Centro de Estudos de Doenças Crónicas (CEDOC), Comprehensive Health Research Centre (CHRC) - pólo NMS |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Medicine (General) medicine.medical_treatment Osteoporosis Adipose tissue Context (language use) tissue expression analysis Carotid endarterectomy 030204 cardiovascular system & hematology Bone remodeling 03 medical and health sciences 0302 clinical medicine R5-920 Internal medicine medicine Original Research Medicine(all) Adiponectin biology business.industry General Medicine medicine.disease osteoporosis 030104 developmental biology Atheroma Endocrinology bone remodeling biomarkers RANKL biology.protein Medicine atherosclerosis business pro inflammatory cytokines |
| Zdroj: | Frontiers in Medicine Frontiers in Medicine, Vol 8 (2021) |
| Popis: | Funding: We wish to thank all the collaborators (administrative staff, nurses, etc.) of the surgery block, as well as the doctors of the vascular surgery and transplantation departments of the Hospital of Santa Maria for the availability and assistance in the collection of the samples. We also thank Sociedade Portuguesa de Reumatologia for funding with two fellowships: Fundo de Apoio à Investigação 2014 and SPR/MSD 2015. DC-F received funding from a PhD grant from Fundação para a Ciência e a Tecnologia (SFRH/BD/80940/2011). Background and Aims: Atherosclerosis and osteoporosis share common risk factors, as well as inflammatory mechanisms. Our aim was to understand how atherosclerotic lesions are related with disturbances in bone. Methods: Gene expression of pro-inflammatory and bone metabolism related proteins (IL-1β, IL-6, IL-17A, TNF, RANKL, OPG, COL1, CTSK, OCL, TRAP, CBFA1, DKK1, SOST, ADIPOQ, and ADIPOR1) were analyzed in arteries and bones from 45 deceased donors and adipose tissue was used as control. Additionally, in 139 patients with advanced atherosclerosis submitted to carotid endarterectomy we compared calcium content (Alizarin red) and plaque inflammatory scores (CD3+, CD68+, and adiponectin) of patients with normal bone mineral density (BMD) with those with low BMD and explored the associations between gene expression in atherosclerotic plaques and BMD. Serum levels of pro-inflammatory and bone related proteins were measured both in donors and patients. Associations were investigated by the Pearson or Spearman correlation tests, and multivariate regression analyzes were performed when justified. Results: Gene expression of bone remodeling and pro-inflammatory proteins correlated positively in bone and aorta, independently of age and sex of donors, but not in adipose tissue. The expression of bone formation genes was significantly higher in atheroma plaques from endarterectomized patients with normal vs. low BMD as well as inflammatory CD68+ scores, regardless of patients' age and sex, but not of body mass index. No relationship was observed between serum levels and gene expression levels of pro-inflammatory or bone remodeling proteins. Conclusions: Our results suggest that the relationship between bones and vessels in the context of atherosclerotic disease and osteoporosis may rely on the intrinsic connection between the tissues involved, independently of disease stage. Serum measurements of pro-inflammatory and bone-remodeling proteins do not accurately translate tissue pathologic processes. publishersversion published |
| Databáze: | OpenAIRE |
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