Histomorphological and immunofluorescence evaluation of clear corneal incisions after microcoaxial phacoemulsification with 2.2 mm and 1.8 mm systems
| Autor: | Kaid Johar, Abhay R. Vasavada, Anshul I. Arora, Viraj A. Vasavada, Mamidipudi R. Praveen |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Collagen i
medicine.medical_specialty Microsurgery genetic structures Descemet membrane medicine.medical_treatment Intraocular lens Collagen Type I Cornea Lens Implantation Intraocular medicine Animals Fluorescent Antibody Technique Indirect Wound Healing Phacoemulsification business.industry Ultrasound eye diseases Sensory Systems Surgery Ophthalmology Microscopy Fluorescence Research centre sense organs Rabbits business |
| Zdroj: | Journal of cataract and refractive surgery. 39(4) |
| ISSN: | 1873-4502 |
| Popis: | Purpose To compare changes in the incision’s histomorphology and denaturation of collagen I in rabbit eyes having microcoaxial phacoemulsification through 2.2 mm and 1.8 mm incision–compatible systems. Design Randomized experimental trial. Setting Iladevi Cataract & IOL Research Centre, Ahmedabad, India. Methods Thirty rabbit eyes were randomized into Group 1 (microcoaxial phacoemulsification through 2.2 mm incisions using Infiniti system [torsional ultrasound]) and Group 2 (microcoaxial phacoemulsification through 1.8 mm incisions using Stellaris system [longitudinal ultrasound]). Each group was then divided into 3 subgroups of 5 eyes each based on 1 of the 3 intervention options: phacoemulsification only, intraocular lens (IOL) insertion only, and phacoemulsification with IOL insertion. Left eyes were randomized for microcoaxial phacoemulsification, and right eyes were treated as controls. Results After phacoemulsification, eyes in Group 1 showed loss of epithelium at the roof of the incisions and Descemet membrane detachment at the floor of the incisions. These findings did not change after IOL insertion. After phacoemulsification, eyes in Group 2 showed loss of epithelium, but Descemet membrane remained attached. There was a longitudinal split in the incision’s stroma in the direction of internal entry. The stromal damage increased after IOL implantation. Immunofluorescence studies showed no obvious irregularities in the arrangement of collagen I in either group. A dot blot analysis showed significant denaturation of collagen I in Group 2. Conclusion The histomorphology of the 2.2 mm system incision showed localized Descemet membrane detachment and endothelial cell loss. The 1.8 mm system incision showed exaggerated stromal damage after IOL insertion. Financial Disclosure No author has a financial or proprietary interest in any material or method mentioned. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|