Fate-mapping the mammalian hindbrain: segmental origins of vestibular projection neurons assessed using rhombomere-specific hoxa2 enhancer elements in the mouse embryo
| Autor: | Joel C. Glover, Carmen Diaz, Jean-Sébastien Renaud, Massimo Pasqualetti, Filippo M. Rijli |
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| Přispěvatelé: | Laboratori di Biologia Cellulare e dello Sviluppo, University of Pisa - Università di Pisa, Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2007 |
| Předmět: |
MESH: Mice
Transgenic Rhombomere MESH: Neurons Hindbrain Embryonic Structures Mice Transgenic Biology MESH: Embryonic Structures 03 medical and health sciences Mice 0302 clinical medicine Species Specificity Fate mapping Genes Reporter MESH: Homeodomain Proteins medicine MESH: Species Specificity Animals MESH: Animals Cell Lineage MESH: Mice 030304 developmental biology Vestibular system Homeodomain Proteins Neurons 0303 health sciences General Neuroscience MESH: Enhancer Elements (Genetics) MESH: Embryo MESH: Genes Reporter MESH: Rhombencephalon [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Articles Ciencias naturales y ciencias de la salud MESH: Cell Lineage Vestibular Nuclei Neuromere Embryo Mammalian Embryonic stem cell Rhombencephalon medicine.anatomical_structure Enhancer Elements Genetic MESH: Vestibular Nuclei Neuron Brainstem Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | RUIdeRA: Repositorio Institucional de la UCLM Universidad de Castilla-La Mancha RUIdeRA. Repositorio Institucional de la UCLM instname Journal of Neuroscience Journal of Neuroscience, Society for Neuroscience, 2007, 27 (36), pp.9670-81. ⟨10.1523/JNEUROSCI.2189-07.2007⟩ |
| ISSN: | 0270-6474 1529-2401 |
| DOI: | 10.1523/JNEUROSCI.2189-07.2007⟩ |
| Popis: | As a step toward generating a fate map of identified neuron populations in the mammalian hindbrain, we assessed the contributions of individual rhombomeres to the vestibular nuclear complex, a major sensorimotor area that spans the entire rhombencephalon. Transgenic mice harboring either thelacZor the enhanced green fluorescent protein reporter genes under the transcriptional control of rhombomere-specificHoxa2enhancer elements were used to visualize rhombomere-derived domains. We labeled functionally identifiable vestibular projection neuron groups retrogradely with conjugated dextran-amines at successive embryonic stages and obtained developmental fate maps through direct comparison with the rhombomere-derived domains in the same embryos. The fate maps show that each vestibular neuron group derives from a unique rostrocaudal domain that is relatively stable developmentally, suggesting that anteroposterior migration is not a major contributor to the rostrocaudal patterning of the vestibular system. Most of the groups are multisegmental in origin, and each rhombomere is fated to give rise to two or more vestibular projection neuron types, in a complex pattern that is not segmentally iterated. Comparison with studies in the chicken embryo shows that the rostrocaudal patterning of identified vestibular projection neuron groups is generally well conserved between avians and mammalians but that significant species-specific differences exist in the rostrocaudal limits of particular groups. This mammalian hindbrain fate map can be used as the basis for targeting genetic manipulation to specific subpopulations of vestibular projection neurons. |
| Databáze: | OpenAIRE |
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