Biocompatible Three-Dimensional Printed Thermoplastic Scaffold for Osteoblast Differentiation of Equine Induced Pluripotent Stem Cells
| Autor: | Arabella Baird, Deborah J. Guest, Aram Saeed, Noelia Dominguez Falcon |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Scaffold
Induced Pluripotent Stem Cells 0206 medical engineering Biomedical Engineering Medicine (miscellaneous) Biocompatible Materials Bioengineering 02 engineering and technology Bone healing Biology Regenerative medicine Mice 03 medical and health sciences Calcification Physiologic Cell Adhesion medicine Animals Humans Horses Induced pluripotent stem cell Cell Proliferation 030304 developmental biology 0303 health sciences Osteoblasts Tissue Scaffolds Temperature Cell Differentiation Osteoblast 3T3 Cells Fibroblasts Biocompatible material 020601 biomedical engineering In vitro Extracellular Matrix Cell biology medicine.anatomical_structure Gene Expression Regulation Printing Three-Dimensional Bone forming |
| Zdroj: | Tissue Engineering Part C: Methods. 25:253-261 |
| ISSN: | 1937-3392 1937-3384 |
| DOI: | 10.1089/ten.tec.2018.0343 |
| Popis: | Horses, like humans, can experience bone fractures and due to their large size and the need to bear weight on all limbs during the recovery period, they can be difficult to treat. Surgical techniques to improve fracture repair are improving, but to date, regenerative medicine technologies to aid fracture healing are not commonly applied in horses. We have previously demonstrated that equine induced pluripotent stem cells (iPSCs) can be differentiated into bone forming osteoblasts in 2D culture. In this study, we report on the use of a thermoplastic, 3D-printed polymer to provide a scaffold for successful, in vitro osteoblast differentiation of equine iPSCs. The scaffold provides a transparent, cost-effective solution to allow the analysis of osteoblast differentiation using live-cell imaging, immunohistochemistry, and quantitative polymerase chain reaction. This in vitro study demonstrates the future feasibility of generating 3D bone constructs through the cell seeding of scaffolds to use in regenerative medicine strategies to improve fracture repair in a relevant, large animal model. |
| Databáze: | OpenAIRE |
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