Modifying influence of enalaprilat on mesangial cell DNA synthesis induced by hydrogen peroxide
| Autor: | G. M. Aber, H. M. Phillips, R. J. D'souza, Richard C. Strange |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
DNA Replication
Male medicine.medical_specialty Enalaprilat Cell Survival Clinical Biochemistry Angiotensin-Converting Enzyme Inhibitors Rats Inbred WKY Biochemistry chemistry.chemical_compound Internal medicine medicine Animals Hydrogen peroxide Platelet-Derived Growth Factor chemistry.chemical_classification Reactive oxygen species Mesangial cell biology DNA synthesis Interleukin-6 Biochemistry (medical) Angiotensin-converting enzyme Hydrogen Peroxide General Medicine Molecular biology Glomerular Mesangium Rats Endocrinology chemistry biology.protein Thymidine Platelet-derived growth factor receptor medicine.drug |
| Zdroj: | Clinica Chimica Acta. 259:137-146 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/s0009-8981(96)06480-7 |
| Popis: | This study examined the effect of the angiotensin converting enzyme (ACE) inhibitor, enalaprilat, on mesangial cell (MC) DNA synthesis induced by H2O2, IL-6 and PDGF. MC were incubated with enalaprilat (2.5-100 mumol/l) alone and together with combinations of H2O2 (3 daily pulses of 10(-6) mol/l), IL-6 (5 ng/ml) and PDGF (10 ng/ml). DNA synthesis was assessed after 72 h using [3H]thymidine (3H-TdR) incorporation. Enalaprilat alone had no effect on MC DNA synthesis. Stimulation of MC by H2O2, PDGF and IL-6 alone resulted in increases in 3H-TdR of 4936.6 +/- 1147.5, 5640.5 +/- 1537.6 and 4413.5 +/- 998.4 cpm, respectively (P0.05 above control). Only 2.5 mumol/l enalaprilat effected a significant reduction in IL-6 and PDGF-induced DNA synthesis. Incubation of MC with H2O2 + PDGF or H2O2 + IL-6 resulted in increases of 3H-TdR of 6471.9 +/- 1785.1 and 5507.2 +/- 1270 cpm, respectively (P0.05 above control). Addition of enalaprilat with either H2O2 + PDGF or H2O2 + IL-6 effected significant reductions in DNA synthesis over the range 2.5-100 mumol/l. These data demonstrate that ACE inhibitors modulate MC DNA synthesis induced by reactive oxygen species. |
| Databáze: | OpenAIRE |
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