Synthesizing, characterizing, and toxicity evaluating of Phycocyanin-ZnO nanorod composites: A back to nature approaches
| Autor: | Hossein Shahbani Zahiri, Morvarid Shafiei, Hadi Maleki, Soheila Davaeifar, Kambiz Akbari Noghabi, Ehsan Hashemi, Mehdi Mohammadi, Mohammad-Hossein Modarresi, Hojatollah Vali |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Thermogravimetric analysis Materials science Administration Oral Metal Nanoparticles Nanoparticle chemistry.chemical_element 02 engineering and technology Zinc 01 natural sciences Cell Line Colloid and Surface Chemistry Differential scanning calorimetry Microscopy Electron Transmission Spectroscopy Fourier Transform Infrared 0103 physical sciences Phycocyanin Zeta potential Animals Physical and Theoretical Chemistry Fourier transform infrared spectroscopy Mice Inbred BALB C Nanotubes 010304 chemical physics Body Weight Surfaces and Interfaces General Medicine Fibroblasts 021001 nanoscience & nanotechnology Anti-Bacterial Agents chemistry Microscopy Electron Scanning Nanorod Zinc Oxide Reactive Oxygen Species 0210 nano-technology Biotechnology Nuclear chemistry |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 175:221-230 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2018.12.002 |
| Popis: | C-Phycocyanin pigment was purified from a native cyanobacterial strain using a novel consecutive multi-step procedure and utilized for the first time for the green synthesis of phycocyanin-zinc oxide nanorods (PHY-ZnO NRs) by a simple, low-cost and eco-friendly approach. The PHY-ZnO NRs were characterized using UV–vis spectroscopy, X-ray diffraction (XRD), zeta potential measurement, FTIR, SEM, TEM, differential scanning calorimetry (DSC), thermogravimetric (TGA), and EDX spectroscopy analysis. The UV–vis spectra showed an absorption band at 364 nm which is characteristic of ZnO nanoparticles (ZnONPs). The rod-shaped PHY-ZnO NRs observed in the TEM and SEM images had an average diameter size of 33 nm, which was in good agreement with the size calculated by XRD. The elemental analysis of PHY-ZnO NRs composition showed that three emission peaks of zinc metal and one emission peak of oxygen comprised 33.88% and 42.50%, respectively. The thermogram of PHY-ZnO NRs sample exhibited the weight loss of biosynthesized nanoparticles registered to be 3%, emphasizing the purity and heat stability of zinc oxide nanorods coated with phycocyanin pigment-protein. MTT assay indicated that PHY-ZnO NRs had a less cytotoxicity on fibroblast L929 compared to the ZnONRs-treated cells. A remarkable increase in ROS level was measured in cells treated with ZnO at final concentrations of 100, 200 and 500 μg/ml (78 ± 7, 99 ± 8 and 116 ± 11, respectively). When it comes to PHY-ZnO NRs, a protective effect for phycocyanin was detected which declined the level of ROS content as confirmed by fluorescent microscopy. The distinctive features of phycocyanin for surface functionalization of ZnO nanoparticles deserve to be deemed as a nano-drug candidate for further researches. |
| Databáze: | OpenAIRE |
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