The Caenorhabditis elegans peb-1 Gene Encodes a Novel DNA-Binding Protein Involved in Morphogenesis of the Pharynx, Vulva, and Hindgut
| Autor: | Laura Beaster-Jones, Christina Haun, Peter G. Okkema, Anthony P. Fernandez, Jack D. Thatcher |
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| Rok vydání: | 2001 |
| Předmět: |
Embryo
Nonmammalian Molecular Sequence Data Biology DNA-binding protein Vulva Sequence Homology Nucleic Acid Gene expression Morphogenesis Animals Protein Isoforms Amino Acid Sequence Binding site Cloning Molecular Enhancer Caenorhabditis elegans Caenorhabditis elegans Proteins Promoter Regions Genetic Transcription factor Gene Molecular Biology Gene Library Base Sequence Sequence Homology Amino Acid Cell Biology biology.organism_classification Molecular biology DNA-Binding Proteins Regulatory sequence Pharynx Female Digestive System Sequence Alignment Developmental Biology |
| Zdroj: | Developmental Biology. 229(2):480-493 |
| ISSN: | 0012-1606 |
| DOI: | 10.1006/dbio.2000.9978 |
| Popis: | Gene expression in the Caenorhabditis elegans pharynx is regulated in part by organ-specific signals, which in the myo-2 gene target a regulatory sequence called the C subelement. C subelement activity requires the organ specification factor PHA-4, a winged-helix transcription factor expressed in all pharyngeal cells. To identify additional factors involved in pharyngeal organogenesis, we performed a yeast one-hybrid screen for C subelement binding proteins. Here we describe the novel factor PEB-1, which is coexpressed with PHA-4 in many pharyngeal cell types, including muscles, epithelial cells, marginal cells, and glands, but is undetectable in the pharyngeal nervous system. PEB-1 is also detected outside the pharynx in cells surrounding the rectum and vulva, as well as in the germ line. Reduction of peb-1 function using RNAi results in morphological defects in the somatic tissues in which peb-1 is expressed. We have mapped the PEB-1 DNA-binding domain to a 158-residue region, which is unrelated to known DNA-binding proteins but shares some sequence similarity to the Drosophila Mod(mdg4) proteins. PEB-1 specifically recognizes a site in the C subelement that partially overlaps the PHA-4 binding site. Both the PEB-1 and the PHA-4 binding sites are necessary for strong C subelement enhancer activity in some cells in which these factors are coexpressed. In contrast the PEB-1 site is dispensable for C subelement activity in pharyngeal neurons. We propose that PEB-1 functions with PHA-4 to activate target gene expression in cells in which they are coexpressed. |
| Databáze: | OpenAIRE |
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