Immunoassay of human muscle enolase subunit in serum: a novel marker antigen for muscle diseases
| Autor: | Takahashi Yuro, Mokuno Kenji, Okagawa Yoko, Suzuki Fujiko, Kato Kanefusa, Shimizu Atsuko |
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| Rok vydání: | 1983 |
| Předmět: |
Macromolecular Substances
Protein subunit Clinical Biochemistry Enolase Biology Cross Reactions Biochemistry Immunoenzyme Techniques chemistry.chemical_compound medicine Humans Muscular dystrophy Creatine Kinase medicine.diagnostic_test Myoglobin Muscles Biochemistry (medical) Skeletal muscle General Medicine medicine.disease Molecular biology medicine.anatomical_structure chemistry Immunoassay Phosphopyruvate Hydratase biology.protein Creatine kinase ATP synthase alpha/beta subunits |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 131(1-2) |
| ISSN: | 0009-8981 |
| Popis: | A sandwich enzyme immunoassay method for measurement of beta subunit of muscle enolase in human serum was developed by use of purified antibodies to enolase beta subunit and beta-D-galactosidase from Escherichia coli as label. The assay was specific to the beta subunit with no cross-reaction with the alpha and gamma subunits of human enolase. The measurable range was from 10 pg to 10 ng per assay tube or 1 to 1000 ng/ml serum. Coefficients of variation within-run and between-run for the assay of serum beta subunit were less than 14%. Normal adult sera contained about 6 ng/ml of the beta subunit, and the levels were significantly elevated in sera of patients with muscular dystrophy and those with myocardial infarction. Serum levels of the beta subunit correlated well with those of creatine phosphokinase, but poorly with those of myoglobin in the same samples. The specific distribution of beta subunit in skeletal muscle and heart was confirmed by measuring the levels in various tissue extracts. |
| Databáze: | OpenAIRE |
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