Reassortment and mutations associated with emergence and spread of oseltamivir-resistant seasonal influenza A/H1N1 viruses in 2005-2009
| Autor: | Yuan-Pin Huang, Je Lo, Ming-Tsan Liu, Chun-Hui Su, Ching-Yuan Yao, Ji-Rong Yang, Ho-Sheng Wu, Yu-Cheng Lin, Yu-Lin Ho, Li-Ching Hsu |
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| Rok vydání: | 2010 |
| Předmět: |
Viral Diseases
Epidemiology viruses Reassortment lcsh:Medicine medicine.disease_cause Influenza A Virus H1N1 Subtype Emerging Viral Diseases Influenza A virus lcsh:Science Genetics Multidisciplinary biology virus diseases Antivirals Infectious Diseases Medicine Reassortant Viruses Research Article Molecular Sequence Data Taiwan Hemagglutinin (influenza) Neuraminidase H5N1 genetic structure Antiviral Agents Microbiology Antigenic drift Viral Evolution Infectious Disease Epidemiology Cell Line Dogs Oseltamivir Virology Drug Resistance Viral Influenza Human medicine Animals Humans Epidemics Biology Population Biology lcsh:R Antigenic shift Influenza Emerging Infectious Diseases Mutation Microbial Evolution biology.protein lcsh:Q |
| Zdroj: | PLoS ONE PLoS ONE, Vol 6, Iss 3, p e18177 (2011) |
| ISSN: | 1932-6203 |
| Popis: | A dramatic increase in the frequency of the H275Y mutation in the neuraminidase (NA), conferring resistance to oseltamivir, has been detected in human seasonal influenza A/H1N1 viruses since the influenza season of 2007–2008. The resistant viruses emerged in the ratio of 14.3% and quickly reached 100% in Taiwan from September to December 2008. To explore the mechanisms responsible for emergence and spread of the resistant viruses, we analyzed the complete genome sequences of 25 viruses collected during 2005–2009 in Taiwan, which were chosen from various clade viruses, 1, 2A, 2B-1, 2B-2, 2C-1 and 2C-2 by the classification of hemagglutinin (HA) sequences. Our data revealed that the dominant variant, clade 2B-1, in the 2007–2008 influenza emerged through an intra-subtype 4+4 reassortment between clade 1 and 2 viruses. The dominant variant acquired additional substitutions, including A206T in HA, H275Y and D354G in NA, L30R and H41P in PB1-F2, and V411I and P453S in basic polymerase 2 (PB2) proteins and subsequently caused the 2008–2009 influenza epidemic in Taiwan, accompanying the widespread oseltamivir-resistant viruses. We also characterized another 3+5 reassortant virus which became double resistant to oseltamivir and amantadine. Comparison of oseltamivir-resistant influenza A/H1N1 viruses belonging to various clades in our study highlighted that both reassortment and mutations were associated with emergence and spread of these viruses and the specific mutation, H275Y, conferring to antiviral resistance, was acquired in a hitch-hiking mechanism during the viral evolutionary processes. |
| Databáze: | OpenAIRE |
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