Enterohemolysin operon of Shiga toxin-producing Escherichia coli: a virulence function of inflammatory cytokine production from human monocytes
| Autor: | Ikue Taneike, Hui-Min Zhang, Noriko Wakisaka-Saito, Tatsuo Yamamoto |
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| Rok vydání: | 2002 |
| Předmět: |
Operon
Biophysics Virulence medicine.disease_cause Hemolysin Proteins Biochemistry Hemolysis Monocytes Microbiology Shiga Toxin fluids and secretions Structural Biology Shiga toxin-producing Escherichia coli Genetics medicine Escherichia coli Humans RNA Messenger Molecular Biology Gene Enterohemolysin operon DNA Primers biology Base Sequence Toxin Tumor Necrosis Factor-alpha Escherichia coli Proteins mRNA expression Shiga toxin Hemolysin Cell Biology biochemical phenomena metabolism and nutrition bacterial infections and mycoses Interleukin-1β Phenotype Human monocyte biology.protein bacteria Interleukin-1 |
| Zdroj: | FEBS letters. 524(1-3) |
| ISSN: | 0014-5793 |
| Popis: | Shiga toxin-producing Escherichia coli (STEC) is associated with hemolytic uremic syndrome (HUS). Although most clinical isolates of STEC produce hemolysin (called enterohemolysin), the precise role of enterohemolysin in the pathogenesis of STEC infections is unknown. Here we demonstrated that E. coli carrying the cloned enterohemolysin operon (hlyC, A, B, D genes) from an STEC human strain induced the production of interleukin-1beta (IL-1beta) through its mRNA expression but not tumor necrosis factor-alpha from human monocytes. No IL-1beta release was observed with an enterohemolysin (HlyA)-negative, isogenic E. coli strain carrying a mutation in the hlyA gene. The data suggest that enterohemolysin, a pore-forming toxin, induces the production of IL-1beta, which is one of serum risk markers for HUS. |
| Databáze: | OpenAIRE |
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