DRAQ7 as an Alternative to MTT Assay for Measuring Viability of Glioma Cells Treated With Polyphenols
| Autor: | Pooja Yashod, Richard Gullan, Harcharan K. Rooprai, Sholeh Keshavarz, Patrick Lawrence, Derek Davies, Richard Selway |
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| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
food.ingredient Cell Survival Tetrazolium Salts Antineoplastic Agents Article Flow cytometry Inhibitory Concentration 50 chemistry.chemical_compound food Cell Line Tumor Glioma medicine Humans Anthracyclines MTT assay Viability assay Cytotoxicity medicine.diagnostic_test Polyphenols food and beverages General Medicine medicine.disease Molecular biology Staining Thiazoles Oncology chemistry Grape seed extract Curcumin |
| Zdroj: | Anticancer Res |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.14553 |
| Popis: | BACKGROUND/AIM: The tetrazolium-based MTT cytotoxicity assay is well established for screening putative anti-cancer agents. However, it has limitations including lack of reproducibility with glioma cells treated with polyphenols. The aim of this study was to evaluate whether a flow cytometric assay with the anthraquinone, DRAQ7, was a better alternative than the colorimetric MTT assay for measuring cell viability. MATERIALS AND METHODS: Two glioma cell lines (IPSB-18, U373) and 1 pancreatic cancer cell line (AsPC-1) were treated with 4 polyphenols, namely red grape seed extract, red clover extract, anthocyanin-rich extract and curcumin. Cell viability was assessed using MTT assay and DRAQ7 staining. RESULTS: Limitations of MTT assay included lack of sensitivity and interference with the structure and absorbance spectra of polyphenols. Also, DMSO was toxic to glioma cells. Microscopic observations of cells treated with polyphenols confirmed the range of IC(50) values evaluated by DRAQ7, but not by the MTT assay. CONCLUSION: DRAQ7 is a better alternative than MTT for measuring viability of glioma cells treated with brightly coloured polyphenols. |
| Databáze: | OpenAIRE |
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