RIP60 dimers and multiples of dimers assemble link structures at an origin of bidirectional replication in the dihydrofolate reductase amplicon of Chinese hamster ovary cells
| Autor: | Nicholas H. Heintz, Nathaniel Heintz, Lisa Dailey, Joseph S. Wall, Paul Held, Iris A. Mastrangelo, Paul V. C. Hough |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
DNA Replication
Microscopy Electron Scanning Transmission Protein Conformation Sequence (biology) CHO Cells chemistry.chemical_compound Biopolymers Structural Biology Cricetinae Dihydrofolate reductase Scanning transmission electron microscopy Animals Humans Binding site Molecular Biology Magnesium ion Binding Sites biology Chinese hamster ovary cell Nuclear Proteins DNA Amplicon DNA-Binding Proteins Crystallography Microscopy Electron Tetrahydrofolate Dehydrogenase chemistry biology.protein Nucleic Acid Conformation HeLa Cells |
| Zdroj: | Journal of molecular biology. 232(3) |
| ISSN: | 0022-2836 |
| Popis: | We show assembly of low and high multimers of HeLa cell nuclear protein, RIP60, at the origin of bidirectional replication (OBR) identified by Burhans, Vassilev, Caddle, Heintz and DePamphilis in Chinese hamster ovary cells. RIP60 binds a 5′-ATT-3′ reiterated sequence downstream of the OBR and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Specifically bound structures were studied by conventional electron microscopy (EM) and quantitative scanning transmission electron microscopy (STEM). Dimers and multiples of dimers link the downstream binding site that overlaps a bent DNA sequence and the homologous upstream OBR sequence, looping out 700 bp of intervening DNA. Superposed dimers are found at individual unlinked sites, stabilized presumably through protein-protein interaction, and such superposition appears to occur also in the basic link structure. Along the loop, single crossovers and extended twists are observed by conventional EM. By STEM, loop DNA is laterally compacted, with diameter and mass equivalent to double-duplex DNA strands. Supercoiled 736 bp and 5243 bp circular DNAs assume similar laterally compacted geometries that are mostly absent from relaxed forms. These observations parallel the compacted, interwound superhelices viewed by cryo-electron microscopy in vitrified solutions containing magnesium ions, and provide structural evidence in agreement with that from conventional EM for superhelical tension in RIP60 loop DNA. Loop superhelicity could arise as a topological response to linking and suggests a functional role for link formation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|