Immunochemical study of polysaccharide antigen inStreptococcus sobrinusandStreptococcus downeiwith a cross-reactive monoclonal antibody
| Autor: | Yoshihiro Minato, Fusao Ota, Choji Uchiyama, Hiroyuki Tomotake, Hirohisa Kato, Hiromasa Inoue, K Hirota |
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| Rok vydání: | 1994 |
| Předmět: |
Microbiology (medical)
Antigenicity Molecular Sequence Data Immunology Cross Reactions Streptococcus downei medicine.disease_cause Microbiology Epitope Streptococcus sobrinus Antigen Antibody Specificity medicine Immunology and Allergy Antigens Bacterial Mouth biology medicine.diagnostic_test Polysaccharides Bacterial Antibodies Monoclonal Streptococcus General Medicine biology.organism_classification Antibodies Bacterial Precipitin Tests Infectious Diseases Carbohydrate Sequence Immunoassay biology.protein Antibody Haptens Hapten |
| Zdroj: | FEMS Immunology & Medical Microbiology. 8:213-217 |
| ISSN: | 1574-695X 0928-8244 |
| DOI: | 10.1111/j.1574-695x.1994.tb00445.x |
| Popis: | A monoclonal antibody (mAb h-448) was prepared after cell fusion of mouse myeloma cells (SP2/0-Ag-14) to the spleen cells of mice immunised with serotype h strain (MF25) of Streptococcus downei. The antibody (IgM class) reacted in enzyme immunoassay only with whole cells as well as purified polysaccharide (PS) antigen of Streptococcus sobrinus (types d and g) and Streptococcus downei (serotype h), but not with cells or purified PS antigen from any other serotypes of the mutans group of streptococci. mAb h-448 also quantitatively precipitated in solution with the purified antigens. Competitive hapten inhibition tests demonstrated that beta-methylgalactopyranoside inhibited the reaction most strongly. Although rhamnose also showed a substantial inhibitory effect, the results of this study indicate that the antigenic determinant of the PS antigen has a structure similar to the beta-methylgalactopyranoside molecule. |
| Databáze: | OpenAIRE |
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