Enterolobium contortisiliquum Trypsin Inhibitor (EcTI), a Plant Proteinase Inhibitor, Decreases in Vitro Cell Adhesion and Invasion by Inhibition of Src Protein-Focal Adhesion Kinase (FAK) Signaling Pathways*
| Autor: | Claudia Alessandra Andrade de Paula, Adriana Miti Nakahata, Joana Gasperazzo Ferreira, Paloma Korehisa Maza, Helena B. Nader, Misako U. Sampaio, Maria Luiza Vilela Oliva, Vivien Jane Coulson-Thomas, Erika Suzuki |
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| Přispěvatelé: | Universidade Federal de São Paulo (UNIFESP) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Cell Survival
Integrin Proto-Oncogene Proteins pp60(c-src) Wiskott-Aldrich Syndrome Protein Neuronal Antineoplastic Agents Biochemistry Extracellular matrix Focal adhesion Cell Movement Stomach Neoplasms Cell Line Tumor Cell Adhesion Matrix Metalloproteinase 14 Humans Neoplasm Invasiveness Cell adhesion Molecular Biology Protein Kinase Inhibitors Cell Proliferation biology Cell adhesion molecule Integrin beta1 Fabaceae Cell Biology Cell biology Gene Expression Regulation Neoplastic Focal Adhesion Protein-Tyrosine Kinases Invadopodia Cancer cell Cancer research biology.protein Trypsin Inhibitors Cell Adhesion Molecules Cortactin Signal Transduction |
| Zdroj: | Repositório Institucional da UNIFESP Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| Popis: | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Tumor cell invasion is vital for cancer progression and metastasis. Adhesion, migration, and degradation of the extracellular matrix are important events involved in the establishment of cancer cells at a new site, and therefore molecular targets are sought to inhibit such processes. the effect of a plant proteinase inhibitor, Enterolobium contortisiliquum trypsin inhibitor (EcTI), on the adhesion, migration, and invasion of gastric cancer cells was the focus of this study. EcTI showed no effect on the proliferation of gastric cancer cells or fibroblasts but inhibited the adhesion, migration, and cell invasion of gastric cancer cells; however, EcTI had no effect upon the adhesion of fibroblasts. EcTI was shown to decrease the expression and disrupt the cellular organization of molecules involved in the formation and maturation of invadopodia, such as integrin beta 1, cortactin, neuronal Wiskott-Aldrich syndrome protein, membrane type 1 metalloprotease, and metalloproteinase-2. Moreover, gastric cancer cells treated with EcTI presented a significant decrease in intracellular phosphorylated Src and focal adhesion kinase, integrin-dependent cell signaling components. Together, these results indicate that EcTI inhibits the invasion of gastric cancer cells through alterations in integrin-dependent cell signaling pathways. Universidade Federal de São Paulo, Escola Paulista Med, Dept Biochem & Mol Biol, BR-04044020 São Paulo, Brazil Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04044020 São Paulo, Brazil Universidade Federal de São Paulo, Escola Paulista Med, Dept Biochem & Mol Biol, BR-04044020 São Paulo, Brazil Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04044020 São Paulo, Brazil FAPESP: 2009/53766-5 Web of Science |
| Databáze: | OpenAIRE |
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